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Isolated and characterization of a cDNA encoding ethylene-responsive element binding protein (EREBP)/AP2-type protein, RCBF2, in Oryza sativa L.

文献类型: 外文期刊

作者: Liu, Jin-Ge 1 ; Zhang, Zhen 2 ; Qin, Qiu-Lin 2 ; Peng, Ri-He 2 ; Xiong, Ai-Sheng 3 ; Chen, Jian-Min; Xu, Fang; Zhu, 1 ;

作者机构: 1.Shanghai Acad Agr Sci, Agrobiotechnol Res Ctr, Shanghai 201106, Peoples R China

2.Shanghai Acad Agr Sci, Agrobiotechnol Res Ctr, Shanghai 201106, Peoples R China; Nanjing Agr Univ, Dept Hort, Nanjing 210095, Peoples R China; Yangzhou Univ, Coll Biosci & Biotechnol, Yangzhou 225009, Peoples R China; Fudan Univ, State Key Lab Genet Engn, Sch Life Sci, Shanghai 200433, Peoples R China

3.Shanghai Acad Agr Sci, Agrobiot

关键词: ethylene;ethylene binding protein;rice;transcription factor;yeast one-hybrid;CBF GENE FAMILY;LOW-TEMPERATURE;SIGNAL-TRANSDUCTION;FREEZING TOLERANCE;COLD-ACCLIMATION;HIGH-SALT;ARABIDOPSIS;TRANSCRIPTION;EXPRESSION;DROUGHT

期刊名称:BIOTECHNOLOGY LETTERS ( 影响因子:2.461; 五年影响因子:2.457 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: A transcription factor RCBF2 which interacts with C-repeat/DRE was isolated from Oryza sativa L. by a yeast one-hybrid method. Analysis of the deduced RCBF2 amino acid sequence revealed that RCBF2 contained a conserved ethylene-responsive element binding protein (EREBP)/AP2 domain of 59 amino acids and a potential nuclear localization sequence. RCBF2 showed a high level of homology with other CBF family members only in AP2 domain. Phylogenetic analysis showed that RCBF2 might be different from other eight DRE-binding proteins on evolutionary relationship. The semi-quantitative RT-PCR (s-Q RT-PCR) analysis indicated the expression of RCBF2 gene was induced by cold, dehydration and high-salinity, but not by abscisic acid, and the transcription of RCBF2 gene accumulated primarily in rice immature seeds, growing point and shoots.

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