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A modification of nested PCR method for detection of Enterocytozoon hepatopenaei (EHP) in giant freshwater prawn Macrobrachium rosenbergii

文献类型： 外文期刊

作者： Wang, Yuan 1 ; Zhou, Jinyang 1 ; Yin, Menghe 1 ; Ying, Na 1 ; Xiang, Yang 1 ; Liu, Wenchang 1 ; Ye, Junqiang 4 ; Li, Xincang 1 ; Fang, Wenhong 1 ; Tan, Hongxin 2 ;

作者机构： 1.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Key Lab East China Sea Fishery Resources Exploitat, Minist Agr & Rural Affair, Shanghai, Peoples R China

2.Shanghai Ocean Univ, Shanghai Engn Res Ctr Aquaculture, Shanghai, Peoples R China

3.Shanghai Collaborat Innovat Ctr Cultivating Elite, Shanghai, Peoples R China

4.Fisheries Technol Promot Stn Fengxian Dist, Shanghai, Peoples R China

关键词： microsporidia; Enterocytozoon hepatopenaei; Macrobrachium rosenbergii; nested PCR; spore wall protein

期刊名称：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY （ 影响因子：6.073； 五年影响因子：6.34 ）

ISSN： 2235-2988

年卷期： 2022 年 12 卷

页码：

收录情况： SCI

摘要： The microsporidian Enterocytozoon hepatopenaei (EHP) has become a critical threat to the global shrimp aquaculture industry, thus necessitating early detection by screening. Development of a rapid and accurate assay is crucial both for the active surveillance and for the assessment of shrimp with EHP infection. In the present study, a distinct strain of E. hepatopenaei (EHPMr) was found in Macrobrachium rosenbergii. The SWP1 gene analysis revealed it was a new genotype that differed with the common strain isolated from the Litopenaeus vannamei (EHPLv). A nested SWP-PCR method was modified to fix the bug that the original inner primers could not recognize the EHPMr strain. The redesigned inner primers successfully amplified a product of 182 bp for both the EHPMr strain and the EHPLv strain. The new primers also had good specificity and high sensitivity, which may serve as an alternative for EHP genotyping. This study provided a method for detection of EHP in the biosecurity of Macrobrachium rosenbergii farming, and the developed protocol was proposed for the routine investigation and potential carrier screening, especially for molecular epidemiology.

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