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RNA-Seq Identified Putative Genes Conferring Photosynthesis and Root Development of Melon under Salt Stress

文献类型： 外文期刊

作者： Liu, Tai 1 ; Amanullah, Sikandar 2 ; Xu, Huichun 1 ; Gao, Peng 2 ; Du, Zhiqiang 1 ; Hu, Xixi 1 ; Han, Mo 1 ; Che, Ye 1 ; Zhang, Ling 1 ; Qi, Guochao 1 ; Wang, Di 1 ;

作者机构： 1.Heilongjiang Acad Agr Sci, Daqing Branch, Daqing 163711, Peoples R China

2.Northeast Agr Univ, Key Lab Biol & Genet Improvement Hort Crops Northe, Minist Agr & Rural Affairs, Harbin 150030, Peoples R China

3.Northeast Agr Univ, Coll Hort & Landscape Architecture, Harbin 150030, Peoples R China

关键词： melon; salt stress; photosynthesis; membrane-lipid metabolism; growth inhibition

期刊名称：GENES （ 影响因子：3.5； 五年影响因子：3.9 ）

ISSN：

年卷期： 2023 年 14 卷 9 期

页码：

收录情况： SCI

摘要： Melon is an important fruit crop of the Cucurbitaceae family that is being cultivated over a large area in China. Unfortunately, salt stress has crucial effects on crop plants and damages photosynthesis, membranal lipid components, and hormonal metabolism, which leads to metabolic imbalance and retarded growth. Herein, we performed RNA-seq analysis and a physiological parameter evaluation to assess the salt-induced stress impact on photosynthesis and root development activity in melon. The endogenous quantification analysis showed that the significant oxidative damage in the membranal system resulted in an increased ratio of non-bilayer/bilayer lipid (MGDG/DGDG), suggesting severe irregular stability in the photosynthetic membrane. Meanwhile, root development was slowed down by a superoxidized membrane system, and downregulated genes showed significant contributions to cell wall biosynthesis and IAA metabolism. The comparative transcriptomic analysis also exhibited that major DEGs were more common in the intrinsic membrane component, photosynthesis, and metabolism. These are all processes that are usually involved in negative responses. Further, the WGCN analysis revealed the involvement of two main network modules: the thylakoid membrane and proteins related to photosystem II. The qRT-PCR analysis exhibited that two key genes (MELO3C006053.2 and MELO3C023596.2) had significant variations in expression profiling at different time intervals of salt stress treatments (0, 6, 12, 24, and 48 h), which were also consistent with the RNA-seq results, denoting the significant accuracy of molecular dataset analysis. In summary, we performed an extensive molecular and metabolic investigation to check the salt-stress-induced physiological changes in melon and proposed that the PSII reaction centre may likely be the primary stress target.

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