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Characterization and epitope mapping of monoclonal antibodies against PEDV N protein

文献类型： 外文期刊

作者： Zhao, Yongxiang 1 ; Fan, Baochao 1 ; Xue, Junbiao 3 ; Guo, Rongli 1 ; Li, Jizhong 1 ; Zhou, Jinzhu 1 ; Song, Xu 1 ; Zhang, Xuehan 1 ; Tao, Shengce 3 ; Li, Bin 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Vet Med, Key Lab Vet Biol Engn & Technol, Minist Agr, Nanjing, Peoples R China

2.Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety State, Key Lab Cultivat Base, China, Peoples R China

3.Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Key Lab Syst Biomed, Minist Educ, Shanghai, Peoples R China

4.Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Peoples R China

5.Hebei Agr Univ, Coll Vet Sci, Baoding, Peoples R China

关键词： PEDV; Nucleocapsid; Monoclonal antibodies; Characterization; Epitope mapping

期刊名称：VIROLOGY （ 影响因子：3.7； 五年影响因子：3.2 ）

ISSN： 0042-6822

年卷期： 2023 年 579 卷

页码：

收录情况： SCI

摘要： Porcine epidemic diarrhea virus (PEDV) causes acute diarrhea, vomiting, dehydration and high mortality in neonatal piglets. The nucleocapsid (N) protein of PEDV is a highly conserved protein with strong immunoge-nicity and palys an important role in PEDV diagnosis. However, epitopes on the PEDV N protein have not yet been well characterized. Here, 32 monoclonal antibodies (mAbs) against the PEDV N protein were produced and identified. Six new epitopes were first identified by using a high-throughput epitope mapping method named AbMap. Sequence analysis revealed that among the six epitopes five epitopes were highly conserved among different PEDV strains. We also confirmed that the mAbs derived from the six epitopes of PEDV N protein, have no cross-reactivity with transmissible gastro enteritis virus or porcine delta coronavirus. These mAbs and their defined epitopes will help to understand the N protein structure and immunological characteristics, and to develop a rapid, accurate PEDV diagnosis method.

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