您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Isolation and characterization of a novel temperate bacteriophage infecting Aeromonas hydrophila isolated from a Macrobrachium rosenbergii larvae pond

文献类型： 外文期刊

作者： Lyu, Sunjian 1 ; Xiong, Fulei 1 ; Qi, Tianpeng 2 ; Shen, Weifeng 1 ; Guo, Qi 1 ; Han, Mingming 1 ; Liu, Li 1 ; Bu, Weishao 3 ; Yuan, Julin 4 ; Lou, Bao 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Hydrobiol, State Key Lab Managing Biot & Chem Threats Qual &, 198 Shiqiao Rd, Hangzhou 310021, Zhejiang, Peoples R China

2.Zhejiang Univ, Coll Anim Sci, 866 Yuhangtang Rd, Hangzhou 310058, Zhejiang, Peoples R China

3.Yunhe Cty Qingjiang Ecol Breeding Cooperat, Lishui 310018, Zhejiang, Peoples R China

4.Zhejiang Inst Freshwater Fisheries, Agr Minist Key Lab Hlth Freshwater Aquaculture, Key Lab Fish Hlth & Nutr Zhejiang Prov, 999 South Hangchangqiao Rd, Huzhou 313001, Zhejiang, Peoples R China

关键词： Aeromonas hydrophila; Macrobrachium rosenbergii; Bacteriophage; Biological characterization; Genome

期刊名称：VIRUS RESEARCH （ 影响因子：5.0； 五年影响因子：4.0 ）

ISSN： 0168-1702

年卷期： 2024 年 339 卷

页码：

收录情况： SCI

摘要： Aeromonas hydrophila is an opportunistic pathogen that frequently leads to significant mortality in various commercially cultured aquatic species. Bacteriophages offer an alternative strategy for pathogens elimination. In this study, we isolated, identified, and characterized a novel temperate A. hydrophila phage, designated as P05B. The bacteriophage P05B is a myovirus based on its morphological features, and possesses the capability to lyse A. hydrophila strains isolated from shrimp. The optimal multiplicity of infection (MOI), adsorption rate, latent period, and burst size for phage P05B were determined to be 0.001, 91.7 %, 20 min, and 483 PFU/cell, respectively. Phage P05B displayed stability across a range of temperatures (28-50 degrees C) and pH values (4.0-10.0). Sequence analysis unveiled that the genome of phage P05B comprises 32,302 base pairs with an average G + C content of 59.4 %. A total of 40 open reading frames (ORF) were encoded within the phage P05B genome. The comparative genomic analyses clearly implied that P05B might represent a novel species of the genus Bielevirus under Peduoviridae family. A phylogenetic tree was reconstructed, demonstrating that P05B shares a close evolutionary relationship with other Aeromonas and Aeromonas phages. In conclusion, this study increased our knowledge about a new temperate phage of A. hydrophila with strong lytic ability.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台