您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Lignin metabolism plays an essential role in the formation of corky split vein caused by boron deficiency in 'Newhall' navel orange (Citrus sinensis Osb.)

文献类型： 外文期刊

作者： Zhou, Gaofeng 1 ; Sun, Xiaona 1 ; Zhang, Liping 1 ; Zeng, Xiaoli 5 ; Liu, Guidong 1 ; Sheng, Ou 2 ;

作者机构： 1.Gannan Normal Univ, Coll Navel Orange, Natl Navel Orange Engn Res Ctr, Ganzhou 341000, Peoples R China

2.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Guangzhou 510640, Peoples R China

3.Key Lab South Subtrop Fruit Biol & Genet Resource, Guangzhou 510640, Peoples R China

4.Guangdong Prov Key Lab Trop & Subtrop Fruit Tree, Guangzhou 510640, Peoples R China

5.Hubei Three Gorges Polytech, Coll Med, Yichang 443000, Peoples R China

关键词： Citrus; Leaf vein; Boron deficiency; Lignin biosynthesis; Anatomy; Gene expression

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：4.342； 五年影响因子：4.342 ）

ISSN： 0304-4238

年卷期： 2022 年 294 卷

页码：

收录情况： SCI

摘要： Boron (B) is an essential nutrient for plant development, and its deficiency has been frequently observed in many crops. Corky split vein (CSV) is a typical symptom of B deficiency in citrus, but the mechanism for the formation under B deficiency is unclear. Our work highlighted the role of lignin biosynthesis in the formation of CSV. Two-year-old 'Newhall' navel oranges were grown under B-deficient conditions until CSV symptoms developed. The morphology of CSV was observed by vein separation and anatomical analysis, and results showed that the vascular bundle cells were malformed, and their cell walls were heavily thickened. Interestingly, a phellem layer was clearly observed around the vascular bundle, which has not been reported previously. Moreover, many small, new, and incomplete vascular bundles were found around the original vascular bundle. These results indicate that the formation of CSV caused by B deficiency in citrus can probably be attributed to the accumulation of lignin in the vascular bundle of the veins. Lignin content measurement showed the leaf lignin content was markedly increased under B deficiency stress. Further FTIR spectral analysis showed that the relative absorbance corresponding to characteristic lignin peaks were higher in B deficient leaf than control plants, but the ratio of monolignols did not change. These results indicate that lignin accumulation increased significantly under B deficiency stress, and it promotes the formation of CSV symptoms. The key enzymes of lignin biosynthesis were analyzed and the results suggest that B deficiency activated the activity of PAL, C4H, CCR, and CAD enzymes in citrus leaves, with the exception of 4CL. Similarly, lignin biosynthesis key genes expression levels increased under B deficiency stress, including CsPAL, CsC4H, Cs4CL2, Cs4CL3, CsCCR1, CsCAD2, CsCAD3, and CsCAD5. These results implying that lignin metabolism plays an essential role in the formation of CSV under B deficiency. Taken together, our results provide important insights into the mechanism of CSV formation caused by B deficiency stress in citrus.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台