Molecular characterization, expression profile and association analysis with carcass traits of porcine LCAT gene
文献类型： 外文期刊
作者： Qiao, Mu 2 ; Wu, Hua-Yu; Li, Feng-E 2 ; Jiang, Si-Wen 2 ; Xiong, Yuan-Zhu 2 ; Deng, Chang-Yan 1 ;
作者机构： 1.Huazhong Agr Univ, Coll Anim Sci, Wuhan 430070, Peoples R China
2.Huazhong Agr Univ, Key Lab Pig Genet & Breeding, Minist Agr, Wuhan 430070, Peoples R China
3.Huazhong Agr Univ, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ, Wuhan 430070, Peoples R China
4.Huazhong Agr Univ, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ, Wuhan
关键词： swine.;LCAT;SNP;Association analysis;Promoter prediction;Internet resource.
期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）
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收录情况： SCI
摘要： The lecithin cholesterol acyltransferase gene (LCAT) plays an important role in lipoprotein metabolism, especially in the process termed reverse cholesterol transport'. In this study, we obtained the 1,434 bp mRNA sequence of porcine LCAT including the full coding region and encoding a protein of 472 amino acids. The sequence was deposited into the GenBank under the accession no. EU717835. The genomic sequence of this gene which contains six exons and five introns, is 3,712 bp in length (GQ379050). Bioinformatic analysis of the 5o regulatory region has revealed that some transcription factor Sp1, AP-1, AP-2 and NF-kappaB were represented in this region. Tissue expression analysis showed that the porcine LCAT gene is ubiquitously expressed in all examined tissues. Phylogenetic tree was constructed by aligning the amino acid sequences of different species. Moreover, we found a single nucleotide polymorphism (SNP, C/G266) in intron 1 of the LCAT gene and association analysis showed that it was significantly associated with ratio of lean to fat (P < 0.05), caul fat weight (P < 0.01), leaf fat weight (P < 0.05), carcass length (P < 0.05) and bone percentage (P < 0.05). Our study will lay the groundwork for the further investigations on the detailed physiological function of LCAT in pig models.
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