文献类型： 外文期刊

作者： Wang Wei ¹ ; Yang Hong-Lan ¹ ; Bao HuiFang ¹ ; Zhang Daoyuan ² ; Shan Qi-mu-ge ¹ ; Wood, Andrew J. ⁵ ;

作者机构： 1.Xinjiang Acad Agr Sci, Inst Microbiol, Urumqi 830091, Peoples R China

2.Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Urumqi 830011, Peoples R China

3.Xinjiang Engn Res Ctr Microbiol Extreme Habitat, Urumqi 830091, Peoples R China

4.Shehezi Univ, Key Lab Agr Technol, Xinjiang 832003, Peoples R China

5.So Illinois Univ, Coll Sci Plant Biol, Carbondale, IL 62901 USA

关键词： recombinant DNA;Candida utilis.;Expression of xylanase;18S rDNA;Xylanase activity;Bioreactor;Internet resource.

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

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收录情况： SCI

摘要： In order to test whether 18S rDNA can influence positively xylanase gene effective expression in the yeast of Candida utilis, a targeting vector pGLR9K-XA was constructed by adding an interested gene xynA from Streptomyces olivaceoviridis into the vector pGLR9K which is constructed by ourselves. pGLR9K contains the 18S rDNA, GAP promoter and CYH resistance gene sequence, all of which is from C. utilis. Then the vector pGLR9K-XA was transformed into C. utilis. To test the vector and transformed system, PCR, Southern blot and DNS methods were used. The results showed that xylanase gene can be detected in the chromosome DNA of recombinant C. utilis and the enzyme activity of xylanase is up to 60 IU mlp# in the study. It is suggested that this system can be used to express exogenous genes in C. utilis as a bioreactors. This is the first report that xylanase gene was expressed in C. utilis.