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Engineering bacterium for biofilm formation and L-lysine production in continuous fermentation

文献类型： 外文期刊

作者： Peng, Xiwei 1 ; Zhang, Di 1 ; Yuan, Jingyi 1 ; Yang, Hongdan 1 ; Li, Mengting 1 ; Zhang, Huifang 1 ; Niu, Huanqing 1 ; Zhu, Chenjie 1 ; Chen, Yong 1 ; Zhao, Chunguang 2 ; Guo, Ting 3 ; Wang, Zhenyu 1 ; Liu, Dong 1 ; Ying, Hanjie 1 ;

作者机构： 1.Nanjing Tech Univ, Coll Biotechnol & Pharmaceut Engn, State Key Lab Mat Oriented Chem Engn, 30, Puzhu South Rd, Nanjing 211816, Peoples R China

2.Ningxia Eppen Biotech Co Ltd, Yinchuan 750100, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Pomol, 50, Zhongling St, Nanjing 210014, Peoples R China

关键词： Microbial biofilms; Biofilm-enhanced fermentation; C. glutamicum; Gene deletion and expression

期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：9.0； 五年影响因子：9.5 ）

ISSN： 0960-8524

年卷期： 2024 年 414 卷

页码：

收录情况： SCI

摘要： Microbial biofilms provide advantages in fermentation processes. However, Corynebacterium glutamicum (C. glutamicum) usually exhibits relatively poor biofilm formation compared to other industrial strains. To develop a biofilm-enhanced fermentation process for C. glutamicum, seven genes potentially related to biofilm formation in C. glutamicum were systematically investigated, which include ppk2B, glgC, virB11, cslA, NCgl2909, NCgl0350 and exeR. Deletion of the NCgl0350, NCgl2909 genes and heterologous expression of the cslA gene were found to increase biofilm amounts by 16.9%, 21.2% and 135%, respectively, compared to the wild-type strain. Meanwhile, the production of L-lysine by engineered strains was assessed in biofilm-based continuous fermentation. The most notable result was observed for the cslA-expressing strain, which produced an average of 26.1% higher L-lysine compared with that of wild-type strain in 6-L bioreactors. In conclusion, this study offers valuable insights into the biofilm formation in C. glutamicum and its application in continuous fermentation processes.

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