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Identification of a novel angiogenin inhibitor 1 and its association with hyaluronidase of Streptococcus suis serotype 2

文献类型: 外文期刊

作者: Wo, Tao 2 ; Yuan, Fangyan 3 ; Chang, Haitao 3 ; Zhang, Lin 4 ; Chen, Guangping 3 ; Tan, Chen 3 ; Chen, Huanchun 1 ; Bei, 1 ;

作者机构: 1.Huazhong Agr Univ, Coll Anim Sci & Vet Med, Lab Anim Infect Dis, State Key Lab Agr Microbiol,Div Anim Pathogens, Wuhan 430070, Hubei, Peoples R China

2.Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan 430023, Peoples R China

3.Huazhong Agr Univ, Coll Vet Med, Wuhan 430070, Peoples R China

4.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Jinzhou 434000, Peoples R China

关键词: Nervous System: Neural Coordination;Infection;Enzymology: Biochemistry and Molecular Biophysics

期刊名称:MICROBIAL PATHOGENESIS ( 影响因子:3.738; 五年影响因子:3.663 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: To characterize the role of hyaluronidase (Hyl) of Streptococcus sins serotype 2 (S sins 2), the hylA gene that encodes Hyl was cloned, expressed and purified A murine brain cDNA library was used to screen for interacting proteins of Hyl Employing the yeast two-hybrid system, a novel murine ribonuclease, angiogenin inhibitor 1 (AI1), which shares 93% homology with porcine AI1, was shown to interact with Hyl in yeast Through co-immunoprecipitation assays, the interaction between AI1 and Hyl was further confirmed Transcription and translation products of the identified cDNA were detected in both normal cells and S suis 2-infected cells AI1 was found to localize mainly in the cytoplasm of 293T cells. These results suggested that the identified protein AI1 might be involved in the pathogenesis of meningitis through interaction with Hyl of S sins 2

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