Isolation from Ochrobactrum anthropi of a novel class II 5-enopyruvylshikimate-3-phosphate synthase with high tolerance to glyphosate
文献类型： 外文期刊
作者： Tian, Yong-Sheng 1 ; Xiong, Ai-Sheng 1 ; Xu, Jing 1 ; Zhao, Wei 1 ; Gao, Feng 1 ; Fu, Xiao-Yan 1 ; Xu, Hu 1 ; Zheng, Jian 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai 201106, Peoples R China
期刊名称：APPLIED AND ENVIRONMENTAL MICROBIOLOGY （ 影响因子：4.792； 五年影响因子：5.26 ）
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收录情况： SCI
摘要： Applying the genomic library construction process and colony screening, a novel aroA gene encoding 5-enopyruvylshikimate-3-phosphate synthase from Ochrobactrum anthropi was identified, cloned, and overexpressed, and the enzyme was purified to homogeneity. Furthermore, site-directed mutagenesis was employed to assess the role of single amino acid residues in glyphosate resistance.
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