文献类型： 外文期刊

作者： Lv, Haimiao ¹ ; Xu, Xiaoling ⁴ ; Wu, Zihui ¹ ; Lin, Yuxin ¹ ; Liu, Yan ⁴ ; Liu, Miaoyu ¹ ; Xu, Linghua ¹ ; Wang, Xiaojie ¹ ; Sun, Nan ¹ ; Abdel-Shafy, Hamdy ⁵ ; Abdelrahman, Mohamed ¹ ; Alsaegh, Aiman A. ⁷ ; Ahmed, Ahmed Ezzat ⁸ ; Yang, Liguo ¹ ; Hua, Guohua ¹ ;

作者机构： 1.Huazhong Agr Univ, Coll Anim Sci & Technol, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ, Wuhan 430070, Peoples R China

2.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen Branch,Genome Anal Lab Minist Agr, Guangdong Lab Lingnan Modern Agr, Shenzhen 518120, Peoples R China

3.Huazhong Agr Univ, Shenzhen Inst Nutr & Hlth, Shenzhen 518038, Peoples R China

4.Beijing Acad Agr & Forestry Sci, Inst Anim Husb & Vet Med, Lab Anim Reprod, Beijing, Peoples R China

5.Cairo Univ, Fac Agr, Dept Anim Prod, El-Gamma St, Giza 12613, Egypt

6.Assiut Univ, Fac Agr, Anim Prod Dept, Asyut, Egypt

7.Umm Al Qura Univ, Fac Appl Med Sci, Dept Lab Med, Mecca, Saudi Arabia

8.King Khalid Univ, Fac Sci, Biol Dept, POB 9004, Abha 61413, Saudi Arabia

9.South Valley Univ, Fac Vet Med, Dept Theriogenol, Qena 83523, Egypt

10.Huazhong Agr Univ, Natl Ctr Int Res Anim Genet Breeding & Reprod, Frontiers Sci Ctr Anim Breeding & Sustainable Prod, Wuhan 430070, Peoples R China

关键词： Lactation; Yes-associated protein1(YAP1); Prolactin; Melatonin; Mammary epithelial cells

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 293 卷

页码：

收录情况： SCI

摘要： Understanding the physiology and molecular mechanisms of lactogenesis is crucial for enhancing mammalian milk production. Yes-associated protein 1 (YAP1) regulated mammary epithelial cell survival during pregnancy, but its role in lactation maintenance remains unclear. We found that YAP1 was highly expressed in mammary gland across specie, with elevated expression levels during murine gestation and lactation, particularly localized in alveoli epithelial cells. In vivo administration of a YAP1 inhibitor impaired murine milk yield, mammary gland weight, alveolar structure, and mammary epithelial cell dynamics. In vitro, YAP1 positively affected mammary epithelial cell growth and the synthesis of triglyceride and alpha-casein. Notably, the primary lactogenesis hormone Prolactin induced cell growth and triglyceride secretion while enhancing YAP1 expression and activity. In contrast, Melatonin inhibited cell growth and triglyceride synthesis, decreasing YAP1 expression and activity. YAP1 knockdown compromised prolactin induced effects, whereas YAP1 overexpression partially rescued cell functions inhibited by melatonin. Finally, Bioinformatics analyses revealed that YAP1 regulated multiple biological processes related to lactogenesis, including cell cycle, apoptosis, endoplasmic reticulum, amino acid transport and biosynthesis, etc. These finding indicated that YAP1 is essential for mammary epithelial cells growth and secretion and played an essential role in the lactating endocrine network by mediating key hormone functions.