Proteomic Analysis of Pollen-Stigma Interaction between Vitis rotundifolia and Vitis vinifera
文献类型： 外文期刊
作者： Wang, Bo 1 ; Wu, Weimin 1 ; Wang, Xicheng 1 ; Wang, Zhuangwei 1 ; Qian, Yaming 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Pomol, Jiangsu Key Lab Hort Crop Genet Improvement, Zhongling St 50, Nanjing, Jiangsu, Peoples R China
关键词： 4D label-free; Euvitis; grape; incompatibility; Muscadinia; pollination
期刊名称：JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE （ 影响因子：1.53； 五年影响因子：1.737 ）
ISSN： 0003-1062
年卷期： 2022 年 147 卷 3 期
页码：
收录情况： SCI
摘要： Muscadine grape (Vitis rotundifolia) is highly resistant to many diseases and insects that attack european grape (Vitis vinifera). However, distant hybridization incompatibility between V. rotundifolia (female) and V. vinifera (male) impedes the utilization of V. rotundifolia in grape breeding. This study used fourth-dimension label-free protein quantitation to detect the key genes and pathways in the V. rotundifolia stigma after self-pollination (V. rotundifolia x V. rotundifolia) and cross-pollination (V. rotundifolia x V. vinifera). A histological analysis showed that pollen tube growth in the stigma of V. rotundifolia was arrested 8 hours after cross-pollination, but not after self-pollination. A proteomic analysis identified 32 differentially expressed proteins (DEPs) in the stigma of V. rotundifolia between self-pollination and cross-pollination. A heatmap analysis grouped these DEPs into four clusters. The top gene ontology terms were ATPase-coupled transmembrane transporter activity, extracellular region, DNA replication, and cellular carbohydrate biosynthetic process. A Kyoto Encyclopedia of Genes and Genomes analysis revealed that these DEPs participated in DNA replication and starch and sucrose metabolism pathways. The downregulated A5AY88, D7TJ35, D7SU26, F6HJI1, and F6GUE7 may have a role in cross incompatibility. This study revealed the cross incompatibility of grapes at histological and proteomic levels.
- 相关文献
作者其他论文 更多>>
-
Regulatory Effects of Diverse DSF Family Quorum-Sensing Signals in Plant-Associated Bacteria
作者：Li, Kaihuai;Ma, Chaoyun;Zhou, Xue;Xiong, Chunlan;Wang, Yong;Liu, Fengquan;Li, Kaihuai;Ma, Chaoyun;Zhou, Xue;Xiong, Chunlan;Wang, Yong;Liu, Fengquan;Li, Kaihuai;Wang, Yong;Wang, Bo;Liu, Fengquan
关键词：bacterial intraspecies regulation; diffusible signal factor (DSF); plant innate immunity; quorum sensing (QS)
-
Comparative analysis of different bio-organic fertilizers on growth and rhizosphere environment of grapevine seedlings
作者：Liu, Siyu;Zhang, Peizhi;Hakeem, Abdul;Niu, Mingxu;Fang, Jinggui;Shangguan, Lingfei;Liu, Siyu;Zhang, Peizhi;Hakeem, Abdul;Fang, Jinggui;Shangguan, Lingfei;Wang, Xicheng;Song, Siyan
关键词：Bio-organic fertilizer; Grapevine seedlings; Rhizosphere environment; Microbial community
-
Inferring the chronic copper deficiency stress responsive network and comparative analysis with excessive copper stress in grapevine
作者：Liu, Siyu;Lin, Yilin;Yang, Yuxian;Xia, Jiaxin;Niu, Mingxu;Hakeem, Abdul;Fan, Huihui;Fang, Jinggui;Shangguan, Lingfei;Liu, Siyu;Lin, Yilin;Yang, Yuxian;Xia, Jiaxin;Hakeem, Abdul;Fang, Xiang;Fan, Huihui;Fang, Jinggui;Shangguan, Lingfei;Fang, Xiang;Wang, Xicheng
关键词：Grapevine; Copper deficiency stress; Leaves; Transcriptomics; Metabolomics
-
Antifungal Compound from the Predatory Bacterium Lysobacter enzymogenes Inhibits a Plant Pathogenic Fungus by Targeting the AAA ATPase VpVeb1
作者：Hou, Rongxian;Liu, Fengquan;Li, Kaihuai;Liu, Fengquan;Guo, Baodian;Zhao, Yangyang;Li, Chaohui;Tang, Bao;Sun, Weibo;Wang, Bo;Chen, Wenchan;Sheng, Cong;Zhao, Yancun;Liu, Fengquan;Kan, Jialiang
关键词：HSAF; Valsa pyri; VpVeb1; AAA ATPase; virulence
-
Iron ions regulate antifungal HSAF biosynthesis in Lysobacter enzymogenes by manipulating the DNA-binding affinity of the ferric uptake regulator (Fur)
作者：Tang, Bao;Wang, Bo;Xu, Zhizhou;Hou, Rouxian;Zhang, Min;Chen, Xian;Liu, Youzhou;Liu, Fengquan;Tang, Bao;Xu, Zhizhou;Hou, Rouxian;Liu, Fengquan
关键词：Lysobacter enzymogenes; biological pesticides; ferric uptake regulator; heat-stable antifungal factor
-
Drought stress in 'Shine Muscat' grapevine: Consequences and a novel mitigation strategy-5-aminolevulinic acid
作者：Yang, Yuxian;Xia, Jiaxin;Jia, Haoran;Lin, Yiling;Liu, Siyu;Ge, Mengqing;Fang, Jinggui;Shangguan, Lingfei;Yang, Yuxian;Xia, Jiaxin;Fang, Xiang;Jia, Haoran;Lin, Yiling;Liu, Siyu;Ge, Mengqing;Fang, Jinggui;Shangguan, Lingfei;Fang, Xiang;Wang, Xicheng;Pu, Yunfeng
关键词：grapevine; drought stress; ALA; transcriptome; metabolome; alleviation
-
Characterization of VvmiR166s-Target Modules and Their Interaction Pathways in Modulation of Gibberellic-Acid-Induced Grape Seedless Berries
作者：Bai, Yunhe;Luo, Linjia;Xuan, Xuxian;Tang, Wei;Qu, Ziyang;Dong, Tianyu;Qi, Ziyang;Yu, Mucheng;Fang, Jinggui;Wang, Chen;Wang, Zhuangwei;Wu, Weimin
关键词：seedless grape; gibberellin; VvmiR166s; HD-Zip III; lignin
