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Development and evaluation of multi-epitope protein p72 (MeP72) for the serodiagnosis of African swine fever

文献类型： 外文期刊

作者： Zhang, Xingxing 1 ; Guo, Jing 1 ; Wang, Lixia 1 ; Li, Zhiyuan 1 ; Liu, Yucheng 2 ; Tian, Lulu 1 ; Xiao, Chencheng 1 ; Li, 1 ;

作者机构： 1.Shihezi Univ, Coll Anim Sci & Technol, Shihezi 832003, Xinjiang, Peoples R China

2.Xinjiang Acad Agr & Reclamat Sci, Inst Anim Sci & Vet Res, Shihezi 832000, Xinjiang, Peoples R China

3.Chinese Acad Agr Sci, State Key Lab Vet Etiol Biol, Lanzhou Vet Res Inst, Lanzhou 730046, Gansu, Peoples R China

关键词： African swine fever virus; P72 gene; antigenic protein; colloidal gold-based immunochromatographic assay

期刊名称：ACTA VIROLOGICA （ 影响因子：1.162； 五年影响因子：1.255 ）

ISSN： 0001-723X

年卷期： 2021 年 65 卷 3 期

页码：

收录情况： SCI

摘要： African swine fever (ASF) is an acute and severe infectious disease that seriously endangers the global porcine industry. In order to develop ASF serodiagnostic reagents with high specificity and sensitivity, in the present study, the antigenic epitopes of P72 protein of African swine fever virus (ASFV) were analyzed, and the ASFV multi-epitope fusion gene MeP72 in tandem with the dominant linear epitopes was constructed. The recombinant multi-epitope fusion MeP72 (reMeP72) was prepared in Escherichia coli. A colloidal gold-based immunochromatographic assay (CGIA) based on reMeP72 was developed for the detection of antibodies against ASFV. A total of 139 pig clinical serum samples were used for assessment of the potential diagnostic value of reMeP72. The results showed that CGIA did not cross-react with positive sera of viruses, such as classical swine fever virus (CSFV), porcine circovirus type 2 (PCV2), pseudorabies virus (PRV), porcine reproductive and respiratory syndrome virus (PRRSV) and swine influenza virus (SIV), showing high specificity. Sensitivity analysis showed that CGIA could detect ASFV-positive serum at a dilution of 1:64. Compared with commercial ASFV kits, the sensitivity and specificity of ASFV CGIA based on reMeP72 protein were 85.7% and 97.6%, respectively. The agreement rate of the two methods was 96.4%, showing a good detection performance. The results indicated that the reMeP72 was of potential value for the serodiagnosis of ASF.

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