文献类型： 外文期刊

作者： Fang, Cheng ¹ ; Ma, Wei ² ; Zhu, Xiaotong ¹ ; Peng, Lingling ¹ ; Tao, Liangzhi ¹ ; Zhao, Zhiqiang ⁴ ; Wang, Fengbin ⁴ ; Ye, Yafeng ¹ ; Ma, Bojun ³ ; Wu, Yuejin ¹ ; Yuan, Jie ⁴ ; Liu, Binmei ¹ ; Chen, Xifeng ³ ; Cheng, Weimin ¹ ;

作者机构： 1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei, Peoples R China

2.Huaihe River Basin Ecol Environm Supervis & Adm Bu, Huaihe River Basin Ecoenvironm Monitoring & Sci Re, Bengbu, Peoples R China

3.Zhejiang Normal Univ, Coll & Life Sci, Jinhua, Peoples R China

4.Xinjiang Acad Agr Sci, Inst Crop Sci, Urumqi, Peoples R China

关键词： rice; spl39; lesion-mimic mutant; reactive oxygen species; genomic deletion; disease resistance

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：4.8； 五年影响因子：5.7 ）

ISSN： 1664-462X

年卷期： 2025 年 16 卷

页码：

收录情况： SCI

摘要： Lesion mimic mutants (LMMs) are invaluable for uncovering the molecular mechanisms of programmed cell death (PCD) and plant immunity and identifying more LMMs expands our understanding of these complex processes. In this study, we characterized a novel rice LMM, spl39, identified through heavy-ion beam irradiation. The spl39 mutant exhibits reddish-brown lesions from the tillering stage, reduced plant height, grain size, and fertility. Chloroplast ultrastructure analysis revealed thylakoid disruption and membrane damage, contributing to reduced photosynthetic capacity. Excessive ROS accumulation and reduced antioxidative enzyme activities triggered PCD. Genetic mapping identified a 306-kb deletion in spl39, encompassing 33 genes, including 12 from the diterpenoid biosynthesis gene cluster. Transcriptomic analysis revealed upregulation of hormone signaling and defense-related pathways, consistent with elevated levels of SA, JA, auxins, and cytokinins. The spl39 mutant exhibited enhanced resistance to bacterial blight with reduced lesion lengths and increased expression of defense-related genes. These findings highlight the role of large genomic deletions in reprogramming plant metabolism and immunity, providing new insights into the mechanisms underlying lesion mimic phenotypes and disease resistance in rice.