New Hybrid Strains via Intraspecific Protoplast Fusion of the Entomopathogenic Fungi Lecanicillium spp.
文献类型： 外文期刊
作者： Liu, Liang 1 ; Liu, Chunlai 2 ; Yan, Lili 3 ; Yang, Fan 2 ; Wang, Shuang 2 ; Jiang, Xifeng 2 ; Li, Xinmin 2 ;
作者机构： 1.Heilongjiang Acad Agr Sci, Postdoctoral Programme, Harbin 150086, Heilongjiang, Peoples R China
2.Heilongjiang Acad Agr Sci, Inst Plant Protect IPP, Sci Observing & Expt Stn Crop Pest Harbin, Minist Agr, Harbin 150086, Heilongjiang, Peoples R China
3.Shanghai Univ Engn Sci, Sch Chem & Chem Engn, Shanghai 201620, Peoples R China
关键词： Hybrid strains; L. attenuatum; L. lecannii; pathogenicity; protoplast fusion
期刊名称：SAINS MALAYSIANA （ 影响因子：1.009； 五年影响因子：0.95 ）
ISSN： 0126-6039
年卷期： 2021 年 50 卷 6 期
页码：
收录情况： SCI
摘要： The entomopathogenic fungal genus Lecanicillium Gams and Zare (formerly classified as the species Verticillium lecanii) includes species that are highly pathogenic to many insect genera. In this study, we identified six Lecanicillium spp. isolated strains (designated as V1-V6) belonging to L. lecanii (V1, V3 and V5) and L. attenuatum (V2, V4 and V6). In addition, these strains were used to obtain new strains via protoplast fusion, and nit mutants were used for protoplast selection. Genetic recombination of the hybrid strains was determined using the random amplified polymorphic DNA (RAPD) technique. We obtained nine stable fusant strains from 176 new hybrid strains, which were termed V-12-10, V-14-3, V-16-4, V-23-6, V-25-8, V-34-14, V-36-5, V-45-16 dan V-56-7. Morphological characteristics varied between the hybrid and parental strains. Genomic DNA analysis of the fusants also showed genetic recombination. The median lethal concentration (LC50) for the fusants were lower than that for parental strains, and the median survival time (LT50) for the fusants were reduced compared with that for parental strains. Thus, these results showed that we produced new, more virulent hybrid Lecanicillium spp. strains as biological control agents via intraspecific protoplast fusion.
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