Persulfidation of Nitrate Reductase 2 Is Involved in l-Cysteine Desulfhydrase-Regulated Rice Drought Tolerance
文献类型： 外文期刊
作者： Zhou, Heng 1 ; Zhou, Yin 1 ; Zhang, Feng 1 ; Guan, Wenxue 1 ; Su, Ye 1 ; Yuan, Xingxing 2 ; Xie, Yanjie 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Life Sci, Lab Ctr Life Sci, Nanjing 210095, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Ind Crops, Nanjing 210014, Peoples R China
关键词： hydrogen sulfide; persulfidation; drought stress; nitrate reductase; l-cysteine desulfhydrase
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.924； 五年影响因子：6.132 ）
ISSN：
年卷期： 2021 年 22 卷 22 期
页码：
收录情况： SCI
摘要： Hydrogen sulfide (H2S) is an important signaling molecule that regulates diverse cellular signaling pathways through persulfidation. Our previous study revealed that H2S is involved in the improvement of rice drought tolerance. However, the corresponding enzymatic sources of H2S and its regulatory mechanism in response to drought stress are not clear. Here, we cloned and characterized a putative l-cysteine desulfhydrase (LCD) gene in rice, which encodes a protein possessing H2S-producing activity and was named OsLCD1. Overexpression of OsLCD1 results in enhanced H2S production, persulfidation of total soluble protein, and confers rice drought tolerance. Further, we found that nitrate reductase (NR) activity was decreased under drought stress, and the inhibition of NR activity was controlled by endogenous H2S production. Persulfidation of NIA2, an NR isoform responsible for the main NR activity, led to a decrease in total NR activity in rice. Furthermore, drought stress-triggered inhibition of NR activity and persulfidation of NIA2 was intensified in the OsLCD1 overexpression line. Phenotypical and molecular analysis revealed that mutation of NIA2 enhanced rice drought tolerance by activating the expression of genes encoding antioxidant enzymes and ABA-responsive genes. Taken together, our results showed the role of OsLCD1 in modulating H2S production and provided insight into H2S-regulated persulfidation of NIA2 in the control of rice drought stress.
- 相关文献
作者其他论文 更多>>
-
Two genes encoding caffeoyl coenzyme A O-methyltransferase 1 (CCoAOMT1) are candidate genes for physical seed dormancy in cowpea (Vigna unguiculata (L.) Walp.)
作者：Laosatit, Kularb;Amkul, Kitiya;Somta, Prakit;Lin, Yun;Yuan, Xingxing;Chen, Xin
关键词：
-
Molecular Mechanism of Fusarium Fungus Inhibition by Phenazine-1-carboxamide
作者：Li, Lei;Yin, Mengyu;Yu, Wei;Zou, Jingpei;Ye, Yonghao;Zhang, Feng;Ran, Tingting;Wang, Weiwu;Zhu, Hong;Li, Linwei;Sun, Hao;Guo, Jingjing
关键词：phenazine-1-carboxamide; histone acetyltransferase Gcn5; inhibition; development; pathogenicity; DON production; Fusarium graminearum
-
Identification of a Branch Number Locus in Soybean Using BSA-Seq and GWAS Approaches
作者：Dai, Dongqing;Huang, Lu;Zhang, Xiaoyan;Zhang, Shiqi;Yuan, Yuting;Wu, Gufeng;Hou, Yichen;Yuan, Xingxing;Chen, Xin;Xue, Chenchen
关键词：soybean; branch number; GWAS; BSA; QTL
-
Narrowing down a major QTL region reveals Phytochrome E (PHYE) as the candidate gene controlling flowering time in mungbean (Vigna radiata)
作者：Amkul, Kitiya;Laosatit, Kularb;Yimram, Tarika;Somta, Prakit;Lin, Yun;Chen, Jingbin;Yuan, Xingxing;Chen, Xin
关键词：mungbean; flowering; phytochrome E; QTL; Vigna radiata
-
A Cluster of Peronospora parasitica 13-like (NBS-LRR) Genes Is Associated with Powdery Mildew (Erysiphe polygoni) Resistance in Mungbean (Vigna radiata)
作者：Waengwan, Pitsanupong;Laosatit, Kularb;Yimram, Tarika;Somta, Prakit;Lin, Yun;Yuan, Xingxing;Chen, Xin
关键词：mungbean; Vigna radiata; powdery mildew; Erysiphe polygoni; RPP13
-
Genome-Wide Identification, Expression Analysis, and Potential Roles under Abiotic Stress of the YUCCA Gene Family in Mungbean (Vigna radiata L.)
作者：Wu, Ranran;Chen, Jingbin;Lin, Yun;Jia, Qiyuan;Guo, Yingjian;Liu, Jinyang;Yan, Qiang;Xue, Chenchen;Chen, Xin;Yuan, Xingxing;Wu, Ranran;Chen, Jingbin;Lin, Yun;Liu, Jinyang;Yan, Qiang;Xue, Chenchen;Chen, Xin;Yuan, Xingxing;Jia, Qiyuan;Guo, Yingjian
关键词：mungbean; YUCCA gene family; identification; expression profiles; abiotic stress; transgene
-
Identification and Functional Characterization of WRKY, PHD and MYB Three Salt Stress Responsive Gene Families in Mungbean (Vigna radiata L.)
作者：Li, Shicong;Li, Shicong;Liu, Jinyang;Xue, Chenchen;Lin, Yun;Yan, Qiang;Chen, Jingbin;Wu, Ranran;Chen, Xin;Yuan, Xingxing
关键词：mungbean; transcription factors; WRKY; PHD; MYB; synteny analysis; RNA-seq; differentially expressed genes
