文献类型： 外文期刊

作者： Luo, Fei ¹ ; Duan, Xiaoyu ¹ ; Gao, Ge ¹ ; Zhao, Yaping ¹ ; Li, Meng ¹ ; Qi, Hongyan ¹ ;

作者机构： 1.Shenyang Agr Univ, Coll Hort, Shenyang 110866, Peoples R China

2.Xinjiang Acad Agr Sci, Hami Melon Res Ctr, Nanchang Rd 403, Urumqi 830091, Peoples R China

3.Natl & Local Joint Engn Res Ctr Northern Hort Faci, Key Lab Protected Hort Educ Minist & Liaoning Prov, Shenyang 110866, Peoples R China

关键词： CmWRKY44; (3-carotene; CmERFIV-4; Ethylene; Oriental melon

期刊名称：POSTHARVEST BIOLOGY AND TECHNOLOGY （ 影响因子：6.8； 五年影响因子：7.5 ）

ISSN： 0925-5214

年卷期： 2025 年 222 卷

页码：

收录情况： SCI

摘要： Oriental melon with orange flesh color, a typical climacteric fruit, mainly accumulated (3-carotene. However, the relationship between ethylene and (3-carotene accumulation is still unclear. Here, 'HDB' with orange fleshed melon fruit were treated with ethylene and 1-MCP at 25 days after anthesis (fruit color transformation initial stage). The results showed exogenous ethylene promotes ethylene production, (3-carotene content and up- regulated (3-carotene biosynthesis genes expression. The CmPSY1 promoter characteristics were analyzed, and the transcription factor (TF) CmWRKY44 was identified. Meanwhile, CmWRKY44 located in the nucleus and its expression was up-regulated by ethylene. The EMSA, Y1H, GUS, luciferase reporter assay and the Agrobacterium tumefaciens-mediated transient transformation further indicated CmWRKY44 could bind to CmPSY1 promoter and positively regulate its gene expression. Furthermore, screening yeast one-hybrid library, the CmERFIV-4 was discovered, a nucleus TF, and induced by ethylene. Finally, EMSA, Y1H, luciferase reporter and GUS assay showed that CmERFIV-4 activated the CmWRKY44 gene expression by targeted its promoter. Collectively, exogenous ethylene could accelerate (3-carotene accumulation though cascade of CmERFIV-4 and CmWRKY44 in oriental melon, which provide theoretical basis for carotenoid metabolism in climacteric melon fruit.