文献类型： 外文期刊

作者： Ruberti, Cristina ¹ ; Feitosa-Araujo, Elias ¹ ; Xu, Zhaolong ² ; Wagner, Stephan ¹ ; Grenzi, Matteo ² ; Darwish, Essam ⁵ ; Lichtenauer, Sophie ¹ ; Fuchs, Philippe ¹ ; Parmagnani, Ambra Selene ² ; Balcerowicz, Daria ⁷ ; Schoenaers, Sebastjen ⁷ ; de la Torre, Carolina ⁸ ; Mekkaoui, Khansa ⁹ ; Nunes-Nesi, Adriano ¹⁰ ; Wirtz, Markus ¹¹ ; Vissenberg, Kris ⁷ ; Van Aken, Olivier ⁵ ; Hause, Bettina ⁹ ; Costa, Alex ² ; Schwarzlaender, Markus ¹ ;

作者机构： 1.Univ Munster, Inst Plant Biol & Biotechnol, D-48143 Munster, Germany

2.Univ Milan, Dept Biosci, I-20133 Milan, Italy

3.Jiangsu Acad Agr Sci, Inst Germplasm Resources & Biotechnol, Jiangsu Prov Key Lab Agrobiol, Nanjing 210014, Jiangsu, Peoples R China

4.Univ Bonn, Inst Crop Sci & Resource Conservat INRES, D-53113 Bonn, Germany

5.Lund Univ, Dept Biol, S-22362 Lund, Sweden

6.Cairo Univ, Plant Physiol Sect, Fac Agr, Agr Bot Dept, Giza 12613, Egypt

7.Univ Antwerp, Integrated Mol Plant Physiol Res, B-2020 Antwerp, Belgium

8.Heidelberg Univ, Med Fac Mannheim, NGS Core Facil, D-68167 Mannheim, Germany

9.Leibniz Inst Plant Biochem IPB, Dept Cell & Metab Biol, D-06120 Halle, Saale, Germany

10.Univ Fed Vicosa, Dept Biol Vegetal, BR-36570900 Vicosa, MG, Brazil

11.Heidelberg Univ, Ctr Organismal Studies COS Heidelberg, D-69120 Heidelberg, Germany

12.Hellen Mediterranean Univ, Plant Biochem & Biotechnol Lab, Dept Agr, Iraklion 71410, Greece

13.CNR, Inst Biophys, I-20133 Milan, Italy

14.Univ Milan, Dept Biosci, I-1 Milan, Italy

15.Max Planck Inst Plant Breeding Res, D-50829 Cologne, Germany

16.Andermatt Biocontrol AG, CH-6146 Grossdietwil, Switzerland

期刊名称：PLANT CELL （ 影响因子：12.085； 五年影响因子：12.796 ）

ISSN： 1040-4651

年卷期： 2022 年 34 卷 11 期

页码：

收录情况： SCI

摘要： Ca2+ signaling is central to plant development and acclimation. While Ca2+-responsive proteins have been investigated intensely in plants, only a few Ca2+-permeable channels have been identified, and our understanding of how intracellular Ca2+ fluxes is facilitated remains limited. Arabidopsis thaliana homologs of the mammalian channel-forming mitochondrial calcium uniporter (MCU) protein showed Ca2+ transport activity in vitro. Yet, the evolutionary complexity of MCU proteins, as well as reports about alternative systems and unperturbed mitochondrial Ca2+ uptake in knockout lines of MCU genes, leave critical questions about the in vivo functions of the MCU protein family in plants unanswered. Here, we demonstrate that MCU proteins mediate mitochondrial Ca2+ transport in planta and that this mechanism is the major route for fast Ca2+ uptake. Guided by the subcellular localization, expression, and conservation of MCU proteins, we generated an mcu triple knockout line. Using Ca2+ imaging in living root tips and the stimulation of Ca2+ transients of different amplitudes, we demonstrated that mitochondrial Ca2+ uptake became limiting in the triple mutant. The drastic cell physiological phenotype of impaired subcellular Ca2+ transport coincided with deregulated jasmonic acid-related signaling and thigmomorphogenesis. Our findings establish MCUs as a major mitochondrial Ca2+ entry route in planta and link mitochondrial Ca2+ transport with phytohormone signaling. Monitoring of subcellular Ca2+ dynamics in living Arabidopsis roots reveals that MCU proteins provide the dominant mitochondrial Ca2+ uptake mechanism in vivo.