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The novel hydrolase IpcH initiates the degradation of isoprocarb in a newly isolated strain Rhodococcus sp. D-6

文献类型： 外文期刊

作者： Zhu, Qian 1 ; Liu, Hongfei 1 ; Pan, Kaihua 1 ; Zhu, Wanhe 1 ; Qiao, Yihui 1 ; Li, Qian 1 ; Hu, Junqiang 1 ; Zhang, Mingliang 1 ; Qiu, Jiguo 1 ; Yan, Xin 1 ; Ge, Jing 2 ; Hong, Qing 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Life Sci, Dept Microbiol, Key Lab Agr & Environm Microbiol,Minist Agr & Rura, Nanjing 210095, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Nanjing 210014, Peoples R China

关键词： Isoprocarb; Biodegradation; Rhodococcus sp. D-6; Hydrolase IpcH; Bioremediation

期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：12.2； 五年影响因子：11.9 ）

ISSN： 0304-3894

年卷期： 2024 年 476 卷

页码：

收录情况： SCI

摘要： Isoprocarb (IPC), a representative monocyclic carbamate insecticide, poses risks of environmental contamination and harm to non-target organisms. However, its degradation mechanism has not been reported. In this study, a newly IPC-degrading strain D-6 was isolated from the genus Rhodococcus, and its degradation characteristics and pathway of IPC were analyzed. A novel hydrolase IpcH, responsible for hydrolyzing IPC to 2-isopropylphenol (IPP), was identified. IpcH exhibited low similarity (< 27 %) with other reported hydrolases, including previously characterized carbamate insecticides hydrolases, indicating its novelty. The K-m and k(cat) values of IpcH towards IPC were 69.99 +/- 8.33 mu M and 95.96 +/- 4.02 s(-1), respectively. Also, IpcH exhibited catalytic activity towards various types of carbamate insecticides, including monocyclic carbamates (IPC, fenobucarb and propoxur), bicyclic carbamates (carbaryl and carbofuran), and linear carbamates (oxamyl and aldicarb). The molecular docking and site-directed mutagenesis revealed that His254, His256, His329 and His376 were essential for IpcH activity. Strain D-6 can effectively reduce the toxicity of IPC and IPP towards sensitive organisms through its degradation ability. This study presents the initial report on IPC degradation pathway and molecular mechanism of IPC degradation, and provides a good potential strain for bioremediating IPC and IPP-contaminated environments.

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