Citrobacter rodentium Lysogenized with a Shiga Toxin-Producing Phage: A Murine Model for Shiga Toxin-Producing E. coli Infection
文献类型： 外文期刊
作者： Flowers, Laurice J. 1 ; Hu, Shenglan 1 ; Shrestha, Anishma 1 ; Martinot, Amanda J. 5 ; Leong, John M. 1 ; Osburne, Marcia S. 1 ;
作者机构： 1.Tufts Univ, Sch Med, Dept Mol Biol & Microbiol, Boston, MA 02111 USA
2.Tufts Univ, Grad Sch Biomed Sci, Boston, MA 02111 USA
3.Univ Penn, Dept Dermatol, Philadelphia, PA 19104 USA
4.Minist Agr & Rural Affairs, Guangdong Acad Agr Sci, Inst Anim Sci,Key Lab Anim Nutr & Feed Sci South, State Key Lab Livestock & Poultry Breeding,Guangd, Guangzhou, Peoples R China
5.Tufts Cummings Sch Vet Med, Dept Infect Dis & Global Hlth, North Grafton, MA USA
关键词： Citrobacter rodentium; Shiga toxin; Mouse infection; Stx phage; Lysogeny
期刊名称：SHIGA TOXIN-PRODUCING E. COLI: Methods and Protocols
ISSN： 1064-3745
年卷期： 2021 年 2291 卷
页码：
收录情况： SCI
摘要： Shiga toxin-producing E. coli (STEC) is a common foodborne pathogen in developed countries. STEC generates "attaching and effacing" (AE) lesions on colonic epithelium, characterized by effacement of microvilli and the formation of actin "pedestals" beneath intimately attached bacteria. In addition, STEC are lysogenized with a phage that, upon induction, can produce potent Shiga toxins (Stx), potentially leading to both hemorrhagic colitis and hemolytic uremic syndrome. Investigation of the pathogenesis of this disease has been challenging because STEC does not readily colonize conventional mice. Citrobacter rodentium (CR) is a related mouse pathogen that also generates AE lesions. Whereas CR does not produce Stx, a murine model for STEC utilizes CR lysogenized with an E. coli-derived Stx phage, generating CR(Phi stx), which both colonizes conventional mice and readily gives rise to systemic disease. We present here key methods for the use of CR(Phi stx) infection as a highly predictable murine model for infection and disease by STEC. Importantly, we detail CR(Phi stx) inoculation by feeding, determination of pathogen colonization, production of phage and toxin, and assessment of intestinal and renal pathology. These methods provide a framework for studying STEC-mediated systemic disease that may aid in the development of efficacious therapeutics.
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