Molecular cloning and functional analysis of polymeric immunoglobulin receptor, pIgR, gene in mandarin fish Siniperca chuatsi
文献类型： 外文期刊
作者： Ji, Jia Xiang 1 ; Zhang, Lin 3 ; Li, Li 1 ; Wang, Kai Lun 1 ; Hou, Jing 4 ; Liu, Lan Hao 4 ; Li, Bo 4 ; Zhang, Bai Dong 4 ; Li, Nan 1 ; Chen, Shan Nan 1 ; Nie, Pin 1 ;
作者机构： 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Inst Hydrobiol, Chinese Acad Sci, Key Lab Aquaculture Dis Control, Wuhan 430072, Hubei, Peoples R China
3.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wudayuan First Rd 8, Wuhan 430023, Hubei, Peoples R China
4.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Shandong, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
关键词： pIgR; Ig; Mandarin fish; Siniperca chuatsi; Expression analysis
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）
ISSN： 1050-4648
年卷期： 2023 年 137 卷
页码：
收录情况： SCI
摘要： Polymeric immunoglobulin receptor (pIgR) can bind and transport immunoglobulins (Igs), thus playing a role in mucosal immunity. In this study, pIgR gene was cloned in mandarin fish, Siniperca chuatsi, with the open reading frame (ORF) of 1011 bp, encoding 336 amino acids. The pIgR protein consists of a signal peptide, an extracellular domain, a transmembrane domain and an intracellular region, with the presence of two Ig-like domains (ILDs) in the extracellular domain, as reported in other species of fish. The pIgR gene was expressed in all organs/tissues of healthy mandarin fish, with higher level observed in liver and spleen. Following the immersion infection of Flavobacterium columnare, pIgR transcripts were detected in immune related, especially mucosal tissues, with significantly increased transcription during the first two days of infection. Through transfection of plasmids expressing pIgR, IgT and IgM, pIgR was found to be interacted with IgT and IgM as revealed by coimmunoprecipitation and immunofluorescence.
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