文献类型： 外文期刊

作者： Wang, Mingcheng ¹ ; Zhong, Jie ⁴ ; Guo, Yanan ⁵ ; Zhao, Shuqiang ⁶ ; Xia, Huili ⁴ ; Wang, Gailing ⁴ ; Liu, Chaoying ⁴ ; Guo, Aizhen ¹ ;

作者机构： 1.Natl Key Lab Agr Microbiol, Wuhan 430070, Peoples R China

2.Coll Vet Med, Wuhan 430070, Peoples R China

3.Huazhong Agr Univ, Hubei Hongshan Lab, Wuhan 430070, Peoples R China

4.Huanghuai Univ, Coll Biol & Food Engn, Zhumadian 463000, Peoples R China

5.Ningxia Acad Agr & Forestry Sci, Anim Sci Inst, Yinchuan 750002, Peoples R China

6.Anim Dis Prevent & Quarantine Ctr Zhumadian City, Zhumadian 463000, Peoples R China

关键词： broiler; Sphingomonas; EPI; intestinal histology; intestinal microbiota

期刊名称：ANIMALS （ 影响因子：2.7； 五年影响因子：3.0 ）

ISSN： 2076-2615

年卷期： 2024 年 14 卷 13 期

页码：

收录情况： SCI

摘要： Simple Summary After adding 4 x 105 CFU/mL of Sphingomonas sp. Z392 to drinking water, through the improvement of intestinal histology and flora structure, the digestive and absorption functions of broiler chickens was enhanced, thereby improving their feed utilization and growth performance. Consequently, the final weight of the broilers increased by 4.33%, and the EPI increased by 10.10%, which led to there being more economic benefits from the broilers. Combined with the ammonia-reducing function of this strain, the use of Sphingomonas sp. Z392 in broiler breeding is expected to provide a new strategy for the sustainable development of the broiler industry. Abstract Probiotics are a prominent alternative to antibiotics in antimicrobial-free broiler farming. To assess the effect of Sphingomonas sp. Z392 (isolated and identified) on broiler growth, 600 one-day-old Kebao broiler chickens were randomly divided into a control group and an experimental group. Each group had three replicates, with 100 broiler chickens being raised in each replicate. Regarding the experimental group of broiler chickens, 4.0 x 105 CFU/mL of Sphingomonas Z392 was added to their drinking water. Then, the changes in broiler body weight, the EPI, intestinal histological structure, and gut microbiota were examined. The results show that the supplementation of the broilers' drinking water with 4 x 105 CFU/mL of Sphingomonas Z392 resulted in an increase in the relative abundance of Lactobacillus, Bacteroides, Lachnospiraceae, Aminobacterium, Oribacterium, Christensenellaceae, Faecalibacterium, Barnesiella, Ruminococcus, Parabacteroides, Phascolarctobacterium, Butyricicoccaceae, and Caproiciproducens, which have been reported to be positively correlated with the improved digestion and absorption of broiler chickens. The relative abundance of Odoribacter, Alistipes, Parabacteroides, and Rikenellaceae increased, and these have been reported to be negatively correlated with the occurrence of intestinal diseases. The relative abundance of Campylobacter, Shigella Castellani, Bilophila, Campylobacter, Clostridia, and Anaerotruncus decreased, and these have been reported to be positively correlated with the occurrence of intestinal diseases. At the same time, the following also increased: the integrity of small intestinal villus morphology; the number of goblet cells in small intestinal epithelial cells; the health of the mitochondria in the cytoplasm of jejunal villous epithelial cells; the number of lysosomes in the cytoplasm of goblet cells in the small intestinal epithelium, ileal villous epithelial cells, and mitochondria in the cytoplasm of large intestinal villous epithelial cells; the VH/CD of the ileum; and digestive, absorption, and defense capabilities. In particular, the final weight increased by 4.33%, and the EPI increased by 10.10%. Therefore, the supplementation of broiler drinking water with Sphingomonas generated better economic benefits from the broiler chickens.