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Transcriptome investigation on the multicellular behavior of Bacillus velezensis Bs916 anchoring surfactin

文献类型： 外文期刊

作者： Dong, Fei 1 ; Ren, Shiying 2 ; Liu, Jiachen 2 ; Bilal, Muhammad 2 ; Li, Bin 3 ; Chen, Xin 1 ; Liu, Xuehui 4 ; Zhong, Haijing 2 ; Zang, Shanshan 4 ; Yuan, Mengxian 5 ; Luo, Chuping 2 ; Jiang, Jihong 1 ;

作者机构： 1.Jiangsu Normal Univ, Sch Life Sci, Key Lab Biotechnol Med Plants Jiangsu Prov, Xuzhou 210023, Jiangsu, Peoples R China

2.Huaiyin Inst Technol, Fac Life Sci & Food Engn, Jiangsu Prov Key Construction Lab Probiot Preparat, Huaian 210023, Jiangsu, Peoples R China

3.Jiangsu Acad Agr Sci, Nanjing 210023, Jiangsu, Peoples R China

4.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China

5.Jiangsu Food & Pharmaceut Sci Coll, Huaian 210023, Jiangsu, Peoples R China

关键词： surfactin; biofilm; multicellular behavior; transcriptome; signals

期刊名称：JOURNAL OF APPLIED MICROBIOLOGY （ 影响因子：4.059； 五年影响因子：4.439 ）

ISSN： 1364-5072

年卷期：

页码：

收录情况： SCI

摘要： Aims To provide valuable information for a comprehensive understanding of the multicellular behavior of Bacillus velezensis Bs916 regulated by surfactin and other natural signals by Transcriptome. Methods and results Transcriptomics revealed a distinct effect on gene expression alterations caused by disruption of the surfactin gene cluster(Delta srf) and 100 mu g/ml surfactin addition(Delta srf + SRF). A total of 1573 differential expression genes were identified among Bs916, Delta srf, and Delta srf + SRF and grouped into eight categories based on their expression profiles. RT-qPCR analysis of 30 candidate genes showed high consistency with those of transcriptome. Additionally, the expression of eight candidate genes regulated by surfactin in a dose-dependent manner was revealed by lacZ fusion. Based on the above evidence, we proposed that surfactin can act as an extracellular signal for monitoring biofilm formation in Bs916 by directly regulating the expression of AbrB, DegS-degU, and SinI-SinR, and indirectly regulating the phosphorylation of ComA and Spo0A. Conclusions The biofilm of Delta srf was unable to restore significantly by surfactin addition, combined inclusion of surfactin (SRF), exopolysaccharide (EPS), and gamma-poly-dl-glutamic acid (gamma-PGA), results in significant restoration of Delta srf biofilm formation, thereby a preliminary model was presented about the molecular mechanism by which the signaling molecule surfactin regulates Bs916 multicellular behavior.

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