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Transcriptomic Changes of Photoperiodic Response in the Hypothalamus Were Identified in Ovariectomized and Estradiol-Treated Sheep

文献类型： 外文期刊

作者： He, Xiaoyun 1 ; Di, Ran 1 ; Guo, Xiaofei 2 ; Cao, Xiaohan 1 ; Zhou, Mei 1 ; Li, Xiaoyu 1 ; Xia, Qing 1 ; Wang, Xiangyu 1 ; Zhang, Jinlong 2 ; Zhang, Xiaosheng 2 ; Liu, Qiuyue 1 ; Chu, Mingxing 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Anim Sci, Key Lab Anim Genet & Breeding & Reprod, Minist Agr & Rural Affairs, Beijing, Peoples R China

2.Tianjin Acad Agr Sci, Inst Anim Husb & Vet Med, Tianjin, Peoples R China

关键词： hypothalamus; photoperiodic response; hormone; pathways; mRNAs; lncRNAs

期刊名称：FRONTIERS IN MOLECULAR BIOSCIENCES （ 影响因子：6.113； 五年影响因子：6.122 ）

ISSN：

年卷期： 2022 年 9 卷

页码：

收录情况： SCI

摘要： Accurate timing of seasonal changes is an essential ability for an animal's survival, and the change in the photoperiod is the key factor affecting reproductive seasonality in mammals. Emerging evidence has suggested that multiple hypothalamic genes participate in the photoperiod-induced regulation of reproductive activities in sheep, but the mechanism is still unclear. In this study, we initially examined the plasma level of two major reproductive hormones, namely, follicle-stimulating hormone (FSH) and prolactin (PRL), under different photoperiods in ovariectomized and estradiol-treated (OVX + E-2) sheep using radioimmunoassay (RIA). Of the two hormones, the concentration of PRL significantly increased with the extension of the photoperiod, while FSH showed the opposite trend. Subsequently, an examination of the transcriptomic variation between the short photoperiod (SP) and long photoperiod (LP) was conducted. Differential expression analyses and functional annotation showed that several key genes in the insulin secretion (VAMP2, PRKACB, PRKCG, and PLCB1), GnRH (MAPK13, CGA, CDC42, ATF4, and LHB) pathways, and circadian entrainment (KCNJ5, PER1, GNB2, MTNR1A, and RASD1), as well as numerous lncRNAs, including XR_173257.3, XR_173415.3, XR_001435315.1, XR_001024596.2, and XR_001023464.2, were shown potentially vital for the hypothalamic photoperiodic response. Four of the differentially expressed mRNAs and lncRNAs were validated by qPCR. The constructed mRNA-mRNA interaction networks further revealed that transcripts potentially participated in hypothalamic thyroid hormone synthesis, endocrine resistance, and neuroactive ligand-receptor interactions. The interactome analysis of lncRNAs and their targets implied that XR_173257.3 and its target arylalkylamine N-acetyltransferase (AANAT) and XR_173415.3 and its target TH might participate in the regulation of seasonal reproduction. Together, the changes in reproductive hormones and transcriptome will help to determine the important photoperiod-induced lncRNAs and mRNAs and provide a valuable resource for further research on reproductive seasonality in sheep.

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