Hemolysis-associated release of hemoglobin induces mitochondrial oxidative phosphorylation (OXPHOS) disturbance and aggravates cell oxidative damage in grass carp (Ctenopharyngodon idella)
文献类型： 外文期刊
作者： Yang, Yan 1 ; Li, Ningjing 1 ; Song, Jialing 1 ; Tian, Ye 1 ; Chen, Bing 2 ; Li, Jiangtao 1 ; Lin, Li 1 ; Qin, Zhendong 1 ;
作者机构： 1.Zhongkai Univ Agr & Engn, Coll Anim Sci & Technol, Guangdong Prov Water Environm & Aquat Prod Secur E, Guangzhou Key Lab Aquat Anim Dis & Waterfowl Breed, Guangzhou 510222, Guangdong, Peoples R China
2.Guangdong Acad Agr Sci, Inst Anim Sci, Lab Anim Nutr & Feed Sci South China, Guangdong Key Lab Anim Breeding & Nutr,Minist Agr, Guangzhou 510640, Peoples R China
关键词： Hemolysis; Hemin; Mitochondrial respiratory chain; Ctenopharyngodon idella
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：3.9； 五年影响因子：4.2 ）
ISSN： 1050-4648
年卷期： 2025 年 157 卷
页码：
收录情况： SCI
摘要： The liver is a key site for the removal of cell-free hemin during hemolysis. However, the mechanism underlying liver damage caused by hemolysis in teleost hemolytic disorderss remains unclear. In this study, the hemin incubation of grass carp liver cells (L8824) and phenylhydrazine (PHZ) injection were employed to simulate in vitro and in vivo hemolysis models. The Cell Counting Kit (CCK) assay results of the L8824 cells showed that the hemin caused obvious cell death and exhibited concentration-dependent characteristics. Furthermore, hemin stimulation significantly increased intracellular iron content, markedly enhanced intracellular ROS (reactive oxygen species) production, triggered the activation of genes linked to iron metabolism, and disrupted mitochondrial structural integrity. The quantitative real-time PCR (qRT-PCR) assay and enzyme activity findings indicated that the hemoglobin (Hb) treatment activated the activity and expression of mitochondrial respiratory chain complexes, while the addition of compound inhibitors I, II, and III could rescue hemin-induced cell death. Finally, a hemolysis model was established via intraperitoneal injection of PHZ in the grass carp. Histopathological analysis and in vivo transcriptome data showed that PHZ-induced hemolysis resulted in liver inflammation and iron and collagen fiber buildup. Additionally, immunofluorescence and immunohistochemical data indicated it enhanced the ROS generation, malondialdehyde (MDA), and 4-hydroxy-2-nonenal (4-HNE), destroyed the mitochondria, and up-regulated the transcription of mitochondrial respiratory chain complexes. In summary, the cell-free Hb released during hemolysis increased iron deposition, disrupted iron metabolism homeostasis, and caused oxidative stress. Consequently, this destroyed mitochondria function and ultimately exacerbated cell death.
- 相关文献
作者其他论文 更多>>
-
Identifying the hotspots of nitrate leaching and its key driving factors in the Yellow River Delta using DNDC model
作者：Song, Jiaxin;Chang, Naijie;Ding, Wuhan;Kang, Jiahao;Yi, Xiaopei;Tian, Ye;Li, Hu;Song, Jiaxin;Song, Jiaxin;Tian, Yi;Ding, Wuhan
关键词：Nitrate leaching; GWR; DNDC; Optimal management practice; Yellow river delta
-
Foodborne iron overload induces oxidative stress and causes mitochondrial damage in the liver of grass carp (Ctenopharyngodon idellus)
作者：Huang, Xiaoman;Yang, Yan;Bai, Yanhan;Yang, Shiyi;Zhang, Linpeng;Liu, Lihan;Tao, Junjie;Tu, Chengming;Lin, Li;Qin, Zhendong;Chen, Bing
关键词：iron overload; Lipid peroxidation; Oxidative stress; Mitochondrial damage; Cell death
-
Cell-free hemoglobin released from hemolysis induces programmed cell death through iron overload and oxidative stress in grass carp (Ctenopharyngodon idella)
作者：Song, Jialing;Li, Ningjing;Yang, Yan;Tian, Ye;Lin, Li;Qin, Zhendong;Chen, Bing;Hu, Jiaxiang
关键词：Ctenopharyngodon idella; Hemoglobin; Iron overload; Oxidative damage
-
In vitro and in vivo evaluation of the effects of condensed tannins and catechins monomers on antioxidant and intestinal health of Chinese seabass (Lateolabrax maculatus)
作者：Dong, Ruiqi;Qiu, Jianqiang;Cao, Junming;Huang, Wen;Chen, Bing;Zhao, Hongxia;Sun, Wenhao;Lu, Huijie;Peng, Kai;Dong, Ruiqi;Qiu, Jianqiang;Sun, Wenhao;Loh, Jiun-Yan
关键词：condensed tannins; catechins monomers; Lateolabrax maculatus; antioxidant; intestinal health
-
Nrf2 protects against oxidative damage induced by hemoglobin in the liver of grass carp ( Ctenopharyngodon idella)
作者：Li, Ningjing;Song, Jialing;Yang, Yan;Huang, Xiaoman;Tian, Ye;Lin, Li;Qin, Zhendong;Chen, Bing
关键词：Nrf2; AMPK; Hb; Oxidative damage; Ctenopharyngodon idella
-
Adipocyte Hyperplasia Facilitated Adipose Tissue Expansion to Alleviate Hepatopancreas Injury in Nile Tilapia (Oreochromis niloticus) Fed High-Fat Diet
作者：Tan, Senyue;Wei, Jiamin;Diao, Ailan;Tocher, Douglas R.;Lin, Zeling;Li, Ruixin;Wang, Shuqi;Chen, Cuiying;Tan, Senyue;Wei, Jiamin;Diao, Ailan;Tocher, Douglas R.;Lin, Zeling;Li, Ruixin;Wang, Shuqi;Chen, Cuiying;Chen, Bing
关键词：glucose metabolism; hepatopancreas; high-fat diet; lipid metabolism; mesenteric adipose tissue; Nile tilapia
-
Sodium butyrate mitigates aflatoxin B1 toxicity in largemouth bass
作者：Hou, Dongqiang;Zhao, Hongxia;Peng, Kai;Chen, Bing;Hu, Junru;Zhu, Xifeng;Wang, Guoxia;Cao, Junming;Huang, Wen;Hou, Dongqiang;Zhao, Hongxia;Zhu, Xifeng
关键词：Aflatoxin B1 (AFB1); Sodium butyrate (SB); Largemouth bass; Intestinal microbial composition; Oxidative stress
