Label-free terahertz microfluidic biosensor for sensitive DNA detection using graphene-metasurface hybrid structures
文献类型： 外文期刊
作者： Zhou, Ruiyun 1 ; Wang, Chen 2 ; Huang, Yuxin 1 ; Huang, Kang 3 ; Wang, Yingli 1 ; Xu, Wendao 1 ; Xie, Lijuan 1 ; Ying, Yi 1 ;
作者机构： 1.Zhejiang Univ, Coll Biosyst Engn & Food Sci, 866 Yuhangtang Rd, Hangzhou 310058, Zhejiang, Peoples R China
2.Guangdong Acad Agr Sci, Inst Qual Stand & Monitoring Technol Agroprod, 20 Jinying Rd, Guangzhou 510640, Guangdong, Peoples R China
3.Univ Auckland, Sch Chem Sci, Auckland 1142, New Zealand
关键词： Terahertz spectroscopy; Graphene; Metasurface; Microfluidics; Biosensor; DNA detection
期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：10.618； 五年影响因子：9.323 ）
ISSN： 0956-5663
年卷期： 2021 年 188 卷
页码：
收录情况： SCI
摘要： Metasurface assisted terahertz (THz) real-time and label-free biosensors have attracted intense attention. However, it is still challenging for specific detection of highly absorptive liquid samples with high sensitivity in the THz range. Here, we incorporated graphene with THz metasurface into a microfluidic cell for sensitive biosensing. The proposed THz graphene-metasurface microfluidic platform can effectively reduce the volume of the sample solution and boost the interaction between biomolecules and THz waves, thus enhancing the sensitivity. As a proof of concept, comparative experiments using other three kinds of microfluidic cells (pure microfluidic cell, metasurface-based microfluidic cell and graphene-based microfluidic cell) were conducted to explore and verify the sensing mechanism, which evidences the high sensitivity of delicate sensing based on the hybrid graphene-metasurface THz microfluidic device. Furthermore, to perform biosensing applications on that basis, specific aptamers were modified on the graphene-metasurface, enabling DNA sequences of foodborne pathogen Escherichia coli O157:H7 to be recognized. Based on the THz microfluidic biosensor, 100 nM DNA short sequences can be successfully detected. The sensing results of antibiotics and DNA based on the graphene-metasurface microfluidic biosensor confirm the superiority of the proposed design and considerable promise in THz biosensing. The novel sensing platform provides the merits of enabling highly sensitive, label-free, low-cost, easy to use, reusable, and real-time biosensing, which opens an exciting prospect for nanomaterial-metasurface hybrid structure assisted THz label-free biosensing in liquid environment.
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