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Characterization of the MMP9 Gene and Its Association with Cryptocaryon irritans Resistance Traits in Trachinotus ovatus (Linnaeus, 1758)

文献类型： 外文期刊

作者： Liu, Jun 1 ; Zhu, Ke-Cheng 1 ; Pan, Jin-Min 1 ; Guo, Hua-Yang 1 ; Liu, Bao-Suo 1 ; Zhang, Nan 1 ; Yang, Jing-Wen 1 ; Zhang, Dian-Chang 1 ;

作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab South China Sea Fishery Resources Exploita, Minist Agr & Rural Affairs, 231 Xingang Rd West, Guangzhou 510300, Peoples R China

2.Sanya Trop Fisheries Res Inst, Sanya 572018, Peoples R China

3.Guangdong Prov Engineer Technol Res Ctr Marine Bio, Guangzhou 510300, Peoples R China

关键词： Trachinotus ovatus; MMP9; Cryptocaryon irritans; single nucleotide polymorphisms

期刊名称：GENES （ 影响因子：3.5； 五年影响因子：3.9 ）

ISSN：

年卷期： 2023 年 14 卷 2 期

页码：

收录情况： SCI

摘要： The MMPs are endogenous proteolytic enzymes that require zinc and calcium as cofactors. MMP9 is one of the most complex matrix metalloproteinases in the gelatinase family and has many biological functions. In mammals, mmp9 is thought to be closely associated with cancer. However, studies in fish have rarely been reported. In this study, to understand the expression pattern of the ToMMP9 gene and its association with the resistance of Trachinotus ovatus to Cryptocaryon irritans, the sequence of the MMP9 gene was obtained from the genome database. The expression profiles were measured by qRT-PCR, the SNPs were screened by direct sequencing, and genotyping was performed. The ToMMP9 gene contained a 2058 bp ORF encoding a putative amino acid sequence of 685 residues. The homology of the ToMMP9 in teleosts was more than 85%, and the genome structure of ToMMP9 was conserved in chordates. The ToMMP9 gene was expressed in different tissues of healthy individuals and was highly expressed in the fin, the gill, the liver and the skin tissues. The ToMMP9 expression in the skin of the infected site and its adjacent sites increased significantly after C. irritans infection. Two SNPs were identified in the ToMMP9 gene, and the SNP (+400A/G) located in the first intron was found to be significantly associated with the susceptibility/resistance to C. irritans. These findings suggest that ToMMP9 may play an important role in the immune response of T. ovatus against C. irritans.

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