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Magnetic beads-based double-stranded DNA fluorescent aptasensor biosensor for deoxynivalenol detection

文献类型： 外文期刊

作者： Meng, Jiajia 1 ; Li, Ruijiao 1 ; Chen, Xinyue 1 ; Wang, Xinyi 1 ; Huang, Qingwen 1 ; Nie, Dongxia 1 ; Guo, Dakai 1 ; Zhao, Zhihui 1 ; Han, Zheng 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Agrofood Stand & Testing Technol, 1000 Jingqi Rd, Shanghai 201403, Peoples R China

关键词： Fluorescent aptasensor; Double-stranded DNA; Deoxynivalenol; Magnetic beans; Aptamer

期刊名称：FOOD CHEMISTRY （ 影响因子：9.8； 五年影响因子：9.7 ）

ISSN： 0308-8146

年卷期： 2025 年 473 卷

页码：

收录情况： SCI

摘要： Deoxynivalenol (DON) is one of the most harmful mycotoxins that poses great health threats to human and animals. Herein, a simple and sensitive magnetic beads-based fluorescent biosensor was successfully prepared for detection of DON in cereals. A stable double-stranded DNA (dsDNA, biotin-sDNA+FAM-cDNA/AP) was formed on the surface of streptavidin-coated magnetic beads (SMBs). DON could specifically bind to aptamer probe (AP), thereby releasing cDNA labeled with 6-carboxyfluorescein (FAM) (FAM-cDNA). After magnetic separation, the amount of FAM-cDNA was analyzed to achieve the detection of DON. Under the optimal conditions, the established aptasensor displayed excellent performance for DON analysis with the nice linear relationship (R2 = 0.9972) in the range of 0.1-50 ng/mL, high sensitivity (detection limit of 0.033 ng/mL) and selectivity. The total analysis time including samples preparation was around 200 min. The designed sensor was validated by determining the recoveries (95.48-112.48 %) in real paddy, wheat and maize samples, and its applicability to them was also verified by super-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/ MS). The current study proposed a promising strategy for DON detection, and could easily be extended for the analysis of other targets through the rational design of the sequences of aptamer probe and cDNA.

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