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Mechanisms of HAHV-1 Interaction with Hemocytes in Haliotis diversicolor supertexta: An In Vitro Study

文献类型： 外文期刊

作者： Wei, Mao-Le 1 ; Li, Ya-Nan 2 ; Wang, Jing-Li 1 ; Ma, Cui-Ping 1 ; Kang, Hui-Gang 1 ; Li, Pei-Jun 2 ; Zhang, Xiang 2 ; Huang, Bo-Wen 2 ; Bai, Chang-Ming 2 ;

作者机构： 1.Qingdao Univ Sci & Technol, Qingdao Nucl Acid Rapid Detect Engn Res Ctr, Sino UAE Int Cooperat Joint Lab Pathogen Microorga, Coll Biol Engn,Qingdao Key Lab Nucl Acid Rapid Det, Qingdao 266042, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, State Key Lab Mariculture Biobreeding & Sustainabl, Key Lab Maricultural Organism Dis Control,Minist A, Qingdao 266071, Peoples R China

3.Yancheng Teachers Univ, Coll Ocean & Biol Engn, Yancheng 224007, Peoples R China

4.Qingdao Marine Sci & Technol Ctr, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China

5.Shandong Ctr Technol Innovat Oyster Seed Ind, Qingdao 266105, Peoples R China

关键词： HAHV-1; abalone; hemocytes; transcriptome; pathogen-host interaction

期刊名称：BIOLOGY-BASEL （ 影响因子：3.5； 五年影响因子：4.0 ）

ISSN：

年卷期： 2025 年 14 卷 2 期

页码：

收录情况： SCI

摘要： Haliotid herpesvirus 1 (HAHV-1) causes significant damage to the abalone aquaculture industry. Knowledge of HAHV-1 invasion and host defense mechanisms is limited due to the lack of stable molluscan cell lines. The present study established an in vitro infection model of HAHV-1 using the primary suspension cultures of hemocytes from Haliotis diversicolor supertexta and Haliotis discus hannai. The cytopathic effects of HAHV-1 on adherent-cultured hemocytes of both species were also investigated. The HAHV-1 DNA loads were firstly monitored by means of quantitative PCR during the development of viral infection, and subsequently the mechanism of interaction between HAHV-1 and hemocytes was explored by means of a transcriptome analysis. H. diversicolor supertexta hemocytes exhibited a high degree of susceptibility to HAHV-1, with viral loads reaching a peak of 4.0 x 107 copies/ng DNA. In contrast, no significant replication was observed in H. discus hannai hemocytes. Transcriptome analysis revealed that HAHV-1 evades the host immune response in the early stages of infection, and hijacks the host's energy and redox metabolism to promote its replication at the late stages. Consequently, this study provides a valuable reference point for the investigation of virus-host interaction between HAHV-1 and abalone in vitro.

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