Development of dCAPS markers for sdhB mutants in fungicide-resistant Botrytis cinerea isolates
文献类型： 外文期刊
作者： Zeng, Rong 1 ; Gao, Ping 1 ; Xu, Lihui 1 ; Yuan, Ya'nan 1 ; Han, Yufei 1 ; Tian, Jiaxi 2 ; Wei, Yuhong 2 ; Gao, Shigang 1 ; Song, Zhiwei 1 ; Zhu, Pu 3 ; Dai, Fuming 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Ecoenvironm Protect Res Inst, Shanghai Key Lab Protect Hort Technol, Shanghai 201403, Peoples R China
2.Jiading Dist Agr Technol Extens Serv Ctr, Shanghai 201899, Peoples R China
3.Jinhua Acad Agr Sci, Jinhua 321000, Peoples R China
关键词： Gray mold disease; Resistance; Sensitive; Site mutation; Restriction enzyme digestion
期刊名称：JOURNAL OF PLANT PATHOLOGY （ 影响因子：2.0； 五年影响因子：2.3 ）
ISSN： 1125-4653
年卷期： 2025 年
页码：
收录情况： SCI
摘要： Botrytis cinerea is responsible for gray mold disease in several economically important crops, including strawberry, tomato, grape, and cucumber, establishing it as a globally significant pathogen. Resistance to succinate dehydrogenase inhibitor (SDHI) fungicides has been linked to single-point mutations in the sdhB gene, with nine mutations reported to date. In this study, we developed dCAPS markers that successfully differentiate seven key mutations (P225F/T/L, N230I, and H272R/Y/L) in SdhB by analyzing restriction enzyme digestion patterns. Field validation with B. cinerea isolates from infected strawberries in Shanghai demonstrated high specificity and reliability, with a 100% accuracy compared to sequencing-based validation. This PCR-based method will assist in the continuous monitoring and effective management of SDHI resistance in B. cinerea. Future applications could expand this approach to other fungal pathogens and integrate it with real-time diagnostics for enhanced disease control in both pre- and post-harvest settings.
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