Biocontrol Potential of Rhizospheric Bacillus Strains Against Sclerotinia minor Jagger Causing Lettuce Drop
文献类型： 外文期刊
作者： Xu, Lihui 1 ; Shang, Qinghua 4 ; Nicolaisen, Mogens 5 ; Zeng, Rong 1 ; Gao, Shigang 1 ; Gao, Ping 1 ; Song, Zhiwei 1 ; Dai, Fuming 1 ; Zhang, Jingze 4 ;
作者机构： 1.Shanghai Acad Agr Sci, Inst Ecoenvironm Protect, Shanghai 201403, Peoples R China
2.Shanghai Key Lab Protected Hort Technol, Shanghai 201403, Peoples R China
3.Shanghai Engn Res Ctr Low Carbon Agr SERCLA, Shanghai 201415, Peoples R China
4.Zhejiang Univ, Inst Biotechnol, State Key Lab Rice Biol & Breeding, Minist Agr Key Lab Mol Biol Crop Pathogens & Insec, Hangzhou 310058, Peoples R China
5.Aarhus Univ, Fac Tech Sci, Dept Agroecol, DK-4200 Slagelse, Denmark
关键词：
antagonism;
期刊名称：MICROORGANISMS （ 影响因子：4.2； 五年影响因子：4.6 ）
ISSN：
年卷期： 2025 年 13 卷 1 期
页码：
收录情况： SCI
摘要： Phytopathogenic Sclerotinia minor Jagger causes lettuce drop, a destructive soil-borne disease. As potential biocontrol agents for this disease, 2 of 31 bacterial strains isolated from soil samples from fields containing S. minor Jagger were identified using in vitro antagonistic assays against S. minor Jagger. Bioactivity experiments showed that Bac20 had higher inhibitory activity against S. minor Jagger than Bac45. Based on 16S rRNA sequences and phylogenetic analysis of a combination of sequences from gyrA, rpoB, purH, polC, and groEL, Bac20 and Bac45 were identified as Bacillus velezensis and Bacillus subtilis, respectively. Lipopeptide compounds produced by each strain were identified using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis. Both strains produced three types of lipopeptides, namely surfactins, iturins, and fengycins, whereas Bac20 showed the strongest intensity in its production of iturins, more than that of Bac45. Bac20 inhibited oxalic acid formation in early-stage lettuce leaves infected with S. minor Jagger, delaying pathogen infestation. Greenhouse experiments for controlling lettuce drop demonstrated that inoculation with Bac20 controlled lettuce drop by 71.7%. In conclusion, this study revealed that B. velezensis Bac20 has high potential for use as a biocontrol agent for controlling the lettuce drop caused by S. minor Jagger.
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