文献类型： 外文期刊

作者： Zhang, Xinyue ¹ ; Huang, Haiying ³ ; Sun, Shili ² ; Li, Dongli ¹ ; Sun, Lingli ² ; Li, Qiuhua ² ; Chen, Ruohong ² ; Lai, Xingfei ² ; Zhang, Zhenbiao ² ; Zheng, Xi ¹ ; Wong, Wing-Leung ¹ ; Wen, Shuai ² ;

作者机构： 1.Wuyi Univ, Sch Biotechnol & Hlth Sci, Jiangmen 529020, Peoples R China

2.Tea Res Inst, Guangdong Acad Agr Sci, Guangdong Key Lab Tea Resources Innovat & Utilizat, Guangzhou 510640, Peoples R China

3.Meizhou Acad Agr & Forestry Sci, Tea Res Inst, Meizhou 514071, Peoples R China

4.Int Healthcare Innovat Inst Jiangmen, Jiangmen 529040, Peoples R China

关键词： cell apoptosis; cell-cycle arrest; PI3K; AKT signalling; green tea extract; effect of aging time

期刊名称：MOLECULES （ 影响因子：4.927； 五年影响因子：5.11 ）

ISSN：

年卷期： 2022 年 27 卷 23 期

页码：

收录情况： SCI

摘要： Food extract supplements, with high functional activity and low side effects, play a recognized role in the adjunctive therapy of human colorectal cancer. The present study reported a new functional beverage, which is a type of Chinese Hakka stir-fried green tea (HSGT) aged for several years. The extracts of the lyophilized powder of five HSGT samples with different aging periods were analyzed with high-performance liquid chromatography. The major components of the extract were found to include polyphenols, catechins, amino acids, catechins, gallic acid and caffeine. The tea extracts were also investigated for their therapeutic activity against human colorectal cancer cells, HT-29, an epithelial cell isolated from the primary tumor. The effect of different aging time of the tea on the anticancer potency was compared. Our results showed that, at the cellular level, all the extracts of the aged teas significantly inhibited the proliferation of HT-29 in a concentration-dependent manner. In particular, two samples prepared in 2015 (15Y, aged for 6 years) and 2019 (19Y, aged for 2 years) exhibited the highest inhibition rate for 48 h treatment (cell viability was 50% at 0.2 mg/mL). Further, all the aged tea extracts examined were able to enhance the apoptosis of HT-29 cells (apoptosis rate > 25%) and block the transition of G1/S phase (cell-cycle distribution (CSD) from 30%) population to G2/M phase (CSD from nearly 30% to nearly 10%) at 0.2 mg/mL for 24 h or 48 h. Western blotting results also showed that the tea extracts inhibited cyclin-dependent kinases 2/4 (CDK2, CDK4) and CylinB1 protein expression, as well as increased poly ADP-ribose polymerase (PRAP) expression and Bcl2-associated X (Bax)/B-cell lymphoma-2 (Bcl2) ratio. In addition, an upstream signal of one of the above proteins, phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) signalling, was found to be involved in the regulation, as evidenced by the inhibition of phosphorylated PI3K and AKT by the extracts of the aged tea. Therefore, our study reveals that traditional Chinese aged tea (HSGT) may inhibit colon cancer cell proliferation, cell-cycle progression and promoted apoptosis of colon cancer cells by inactivating PI3K/AKT signalling.