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Development and Validation of Kompetitive Allele-Specific Polymerase Chain Reaction Markers for Seed Protein Content in Soybean

文献类型： 外文期刊

作者： Li, Shuangzhe 1 ; Guo, Chenyijun 1 ; Feng, Xuezhen 1 ; Wang, Jing 1 ; Pan, Wenjing 2 ; Xu, Chang 1 ; Wei, Siming 1 ; Han, Xue 1 ; Yang, Mingliang 1 ; Chen, Qingshan 1 ; Wang, Jinxing 2 ; Hu, Limin 1 ; Qi, Zhaoming 1 ;

作者机构： 1.Northeast Agr Univ, Coll Agr, Natl Key Lab Smart Farm Technol & Syst, Chinese Minist Educ,Key Lab Soybean Biol, Harbin 150030, Peoples R China

2.Heilongjiang Acad Agr Sci, Suihua Branch, Suihua 152052, Peoples R China

关键词： Glycine max; seed protein content; KASP; marker-assisted selection

期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）

ISSN： 2223-7747

年卷期： 2024 年 13 卷 24 期

页码：

收录情况： SCI

摘要： Seed protein content is a critical trait in soybean breeding, as it provides a primary source of high-quality protein for both human consumption and animal feed. This study aimed to enhance molecular marker-assisted selection for high-protein soybean varieties by developing Kompetitive Allele-Specific Polymerase Chain Reaction (KASP) markers targeted at loci associated with seed protein content. Nineteen markers with high genotyping efficacy were identified through screening. Utilizing SN76 (a high-protein line) as the male parent and SN49 and DS1 (both low-protein lines) as female parents, 484 F6 generation individuals from these hybrid combinations were selected to validate the predictive accuracy of the 19 KASP markers. Notably, KASP-Pro-1, KASP-Pro-2, and KASP-Pro-3 effectively distinguished genotypes associated with high and low protein content, with prediction accuracies of 68.4%, 75.0%, and 83.3%, respectively. These results underscore the reliability and practical utility of the selected molecular markers, which are located within the genes Glyma.03G219900, Glyma.14G119000, and Glyma.17G074400, respectively. Haplotype analysis and gene pyramiding indicate that these three genes may influence seed protein content. Consequently, these KASP markers can be effectively integrated into genetic and genomic research on soybean seed protein content as well as into marker-assisted breeding.

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