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Detection of mcr-1-harbouring Escherichia coli by quantum dot labelling of synthetic small peptides mimicking lipopolysaccharide receptors

文献类型： 外文期刊

作者： Wang, Chenghao 1 ; Tang, Biao 2 ; Wu, Jiusheng 7 ; Jin, Xi 1 ; Ke, Shuwen 1 ; Yang, Hua 2 ; Liu, Yuehuan 1 ;

作者机构： 1.Hangzhou Med Coll, Zhejiang key Lab Expt Anim & Nonclin Lab Studies, Hangzhou 310013, Peoples R China

2.State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Zhejiang, Peoples R China

3.Inst Agroprod Safety & Nutr, Hangzhou 310021, Zhejiang, Peoples R China

4.Zhejiang Acad Agr Sci, Hangzhou 310021, Zhejiang, Peoples R China

5.Hangzhou Med Coll, Sch Clin Med, Hangzhou 310053, Peoples R China

6.Hangzhou Med Coll, Engn Res Ctr Novel Vaccine Zhejiang Prov, Hangzhou 310013, Peoples R China

7.Zhejiang Univ, Coll Anim Sci, Hangzhou 310058, Peoples R China

关键词： Lipopolysaccharide; Colistin resistance; mcr-1; Escherichia coli; Immunofluorescence detection; Quantum dot fluorescent labelling

期刊名称：INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS （ 影响因子：10.8； 五年影响因子：7.2 ）

ISSN： 0924-8579

年卷期： 2023 年 62 卷 3 期

页码：

收录情况： SCI

摘要： Overuse of antibiotics and the emergence of multidrug-resistant bacteria has made colistin the last line of defence against complex infections. In previous studies, MCR-1-mediated colistin resistance was mainly detected through PCR or antimicrobial susceptibility testing. However, intuitive detection methods for phenotype are rarely reported. In this study, two small peptide antibodies were constructed for im-munofluorescence detection of mcr-1-harbouring Escherichia coli: one was a small peptide labelled with a quantum dot antibody; and the other was a small peptide labelled with a fluorescein isothiocyanate (FITC) antibody. Whether using FITC or quantum dots, colistin-resistant bacteria in the sample could be qualitatively detected. The assembled antibodies achieved the desired goals in terms of sensitivity, speci-ficity, precision and repeatability. The non-specific problem of sandwich antigen recognition of lipid A binding to small peptides in modified lipopolysaccharide (LPS) was resolved, and this relatively devel-oped immunofluorescence technique standardised the detection process. Together, in addition to PCR, both fluorescent antibodies can be used for immunofluorescent detection of mcr-1-harbouring E. coli.& COPY; 2023 Elsevier Ltd and International Society of Antimicrobial Chemotherapy. All rights reserved.

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