Enhancing the inhibition of cell proliferation and induction of apoptosis in H22 hepatoma cells through biotransformation of notoginsenoside R1 by Lactiplantibacillus plantarum S165 into 20(S/R)-notoginsenoside R2
文献类型： 外文期刊
作者： Wang, Penghui 1 ; Gao, Yansong 2 ; Yang, Ge 2 ; Zhao, Yujuan 2 ; Zhao, Zijian 2 ; Gao, Ge 1 ; Zhao, Lei 1 ; Li, Shengyu 2 ;
作者机构： 1.Changchun Univ Chinese Med, Sch Pharmaceut Sci, Changchun 130117, Peoples R China
2.Jilin Acad Agr Sci, Inst Agrofood Technol, Changchun 130033, Peoples R China
期刊名称：RSC ADVANCES （ 影响因子：3.9； 五年影响因子：3.9 ）
ISSN：
年卷期： 2023 年 13 卷 42 期
页码：
收录情况： SCI
摘要： Notoginsenoside R2 is a crucial active saponin in Panax notoginseng (Burk.) F. H. Chen, but its natural content is relatively low. In this study, we investigated the biotransformation of notoginsenoside R1 to 20(S/R)-notoginsenoside R2 using Lactiplantibacillus plantarum S165, compared the inhibitory effects on cancer cell proliferation and conducted a mechanistic study. Notoginsenoside R1 was transformed using Lactiplantibacillus plantarum S165 at 37 degree celsius for 21 days. The fermentation products were identified using a combination of HPLC, UPLC-MS/MS, and C-13-NMR methods. The inhibition effects of 20(S/R)-notoginsenoside R2 on H22 hepatoma cells were assessed by CCK-8 and TUNEL assays, and the underlying mechanism was investigated by Western blotting. Lactiplantibacillus plantarum S165 could effectively transform notoginsenoside R1 to 20(S/R)-notoginsenoside R2 with a conversion yield of 82.85%. Our results showed that 20(S/R)-notoginsenoside R2 inhibited H22 hepatoma cells proliferation and promoted apoptosis. The apoptosis of H22 hepatoma cells was promoted by 20(S/R)-notoginsenoside R2 through the blockade of the PI3K/AKT/mTOR signaling pathway. The biotransformation method used in this study resulted in the production of 20(S)-notoginsenoside R2 and 20(R)-notoginsenoside R2 from notoginsenoside R1, and the anti-tumor activity of the transformed substance markedly improved.
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