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The effect of gamma and electron beam irradiation on the structural and physicochemical properties of myofibrillar protein and myosin from grass carp

文献类型： 外文期刊

作者： Shi, Gangpeng 1 ; Zhou, Mingzhu 1 ; Wang, Lan 1 ; Xiao, Zihao 3 ; Shi, Liu 1 ; Jiao, Chunhai 1 ; Wu, Wenjin 1 ; Li, Xin 1 ;

作者机构： 1.Hubei Acad Agr Sci, Inst Farm Prod Proc & Nucl Agr Technol, Wuhan 430064, Peoples R China

2.Hubei Univ Technol, Sch Bioengn & Food Sci, Wuhan, Peoples R China

3.Yangtze Univ, Coll Life Sci, Jingzhou, Peoples R China

关键词： electron beam; myofibrillar protein; myosin; gamma-ray irradiation

期刊名称：JOURNAL OF FOOD BIOCHEMISTRY （ 影响因子：2.72； 五年影响因子：2.543 ）

ISSN： 0145-8884

年卷期： 2021 年 45 卷 8 期

页码：

收录情况： SCI

摘要： Myofibrillar protein (MPS) and myosin (MS) from grass carp was irradiated by gamma-ray and electron beam (EB) irradiation with different dose (2, 4, 6, 8, and 10 kGy). The changes in the physicochemical properties (solubility, Ca2+-ATPase activity, total and reactive sulfhydryl content, surface hydrophobicity [S-0-ANS]), and structure of MPS and MS were investigated in the present work. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis revealed that there were degradation and aggregation of MPS and MS caused by irradiation, and the disappearance of myosin heavy chains (MHC) irradiated by EB was earlier than that of irradiated by gamma-ray. As compared with MPS, the extracted MS was more easily destroyed. With the increase of irradiation dose, the particle size, solubility, Ca2+-ATPase activity, and SH content of MPS and MS decreased (p < .05), while the S-0-ANS first increased and then decreased. Two-way analysis of variance results suggested that the degree of protein denaturation depends on the irradiation mode and dose. Compared with gamma-ray irradiation, the EB irradiation had a greater impact on the physicochemical properties of MPS and MS.

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