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The Amino Acid Permease MoGap1 Regulates TOR Activity and Autophagy in Magnaporthe oryzae

文献类型： 外文期刊

作者： Huang, Changli 1 ; Li, Lin 2 ; Wang, Lei 3 ; Bao, Jiandong 2 ; Zhang, Xiaozhi 3 ; Yan, Jiongyi 4 ; Wu, Jiaqi 1 ; Cao, Na 5 ; Wang, Jiaoyu 2 ; Zhao, Lili 2 ; Liu, Xiaohong 2 ; Yu, Xiaoping 1 ; Zhu, Xueming 2 ; Lin, Fucheng 1 ;

作者机构： 1.China Jiliang Univ, Coll Life Sci, Zhejiang Prov Key Lab Biometrol & Inspect & Quara, Hangzhou 310018, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, State Key Lab Managing Biot & Chem Threats Qual &, Key Lab Biotechnol Plant Protect,Minist Agr & Rur, Hangzhou 310021, Peoples R China

3.Zhejiang Agr & Forest Univ, Coll Adv Agr Sci, Hangzhou 310007, Peoples R China

4.Zhejiang Univ, Inst Biotechnol, State Key Lab Rice Biol, Hangzhou 310058, Peoples R China

5.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China

关键词： rice blast; gene knockout; TOR; autophagy; amino acid permease

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 21 期

页码：

收录情况： SCI

摘要： Rice is an important food crop all over the world. It can be infected by the rice blast fungus Magnaporthe oryzae, which results in a significant reduction in rice yield. The infection mechanism of M. oryzae has been an academic focus for a long time. It has been found that G protein, AMPK, cAMP-PKA, and MPS1-MAPK pathways play different roles in the infection process. Recently, the function of TOR signaling in regulating cell growth and autophagy by receiving nutritional signals generated by plant pathogenic fungi has been demonstrated, but its regulatory mechanism in response to the nutritional signals remains unclear. In this study, a yeast amino acid permease homologue MoGap1 was identified and a knockout mutant of MoGap1 was successfully obtained. Through a phenotypic analysis, a stress analysis, autophagy flux detection, and a TOR activity analysis, we found that the deletion of MoGap1 led to a sporulation reduction as well as increased sensitivity to cell wall stress and carbon source stress in M. oryzae. The Delta Mogap1 mutant showed high sensitivity to the TOR inhibitor rapamycin. A Western blot analysis further confirmed that the TOR activity significantly decreased, which improved the level of autophagy. The results suggested that MoGap1, as an upstream regulator of TOR signaling, regulated autophagy and responded to adversities such as cell wall stress by regulating the TOR activity.

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