Identification of two effective sex-specific DNA markers in silver arowana ( Osteoglossum bicirrhosum)
文献类型： 外文期刊
作者： Liu, Yi 1 ; Sun, Jinhui 2 ; Liu, Chao 1 ; Yang, Yexin 1 ; Wang, Yuanyuan 1 ; Mu, Xidong 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst,Minist Agr & Rural, Guangdong Modern Recreat Fisheries Engn Technol Ct, Key Lab Prevent & Control Aquat Invas Alien Specie, Guangzhou 510380, Peoples R China
2.Tianjin Agr Univ, Coll Fisheries, Tianjin 300384, Peoples R China
3.Guangdong Prov Key Lab Aquat Anim Immunol & Sustai, Guangzhou 510380, Peoples R China
关键词： Osteoglossidae; Silver arowana; Sex-specific markers; ZW sex-determination
期刊名称：AQUACULTURE （ 影响因子：3.9； 五年影响因子：4.4 ）
ISSN： 0044-8486
年卷期： 2025 年 596 卷
页码：
收录情况： SCI
摘要： The silver arowana (Osteoglossum bicirrhosum), often referred to as a living fossil, presents significant challenges in sex identification based on morphological characteristics, with its sex determination mechanism remaining poorly understood. This issue poses considerable obstacles to its captive breeding efforts. Given the species' substantial value for both consumption and ornamental purposes, it is a critical candidate for aquaculture. Through genome sequencing, read mapping, and alignment analysis of five male and five female silver arowanas, along with one mixed pool of males, we successfully identified two sex-specific SNPs and confirmed a ZW model of sex determination in this ancient species. These two SNPs demonstrated remarkable accuracy, achieving 100 % success in genetic sex identification, as validated by Sanger sequencing of PCR products from 135 individuals. Both sex-specific SNPs are located approximately 26 kb upstream of the foxl2 gene, indicating a potentially pivotal role for the foxl2 gene in the sex-determination process of the silver arowana. Consequently, we have developed precise molecular markers for sex identification in the silver arowana, significantly enhancing reproductive efficiency and promoting further industrial development. This achievement lays a foundational basis for investigating the sex-determination mechanism of the silver arowana and other osteoglossid fish, thereby advancing aquaculture practices within this species.
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