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Association analysis of DNA methylation and the tissue/developmental expression of the FUT3 gene in Meishan pigs

文献类型： 外文期刊

作者： Yang, Li 1 ; Liu, Lili 1 ; Cheng, Jinhua 2 ; Wu, Zhengchang 1 ; Bao, Wenbin 1 ; Wu, Shenglong 1 ;

作者机构： 1.Yangzhou Univ, Coll Anim Sci & Technol, Key Lab Anim Genet Breeding Reprod & Mol Design Ji, Yangzhou 225009, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Anim Sci, Nanjing, Peoples R China

关键词： DNA methylation; Developmental expression; FUT3 gene; Pig

期刊名称：GENE （ 影响因子：3.5； 五年影响因子：3.3 ）

ISSN： 0378-1119

年卷期： 2023 年 851 卷

页码：

收录情况： SCI

摘要： Porcine alpha-1,3-fucosyltransferase (FUT3), as a member of the fucosyltransferase family, plays an important role in the resistance of the piglet intestine to pathogenic microbial infection. To further investigate the tissue/developmental expression of FUT3 and its regulatory mechanism, we analyzed changes in the expression of FUT3 in the duodenal tissues of Meishan pigs at different ages and found that the expression of FUT3 showed a decreasing trend with increasing age. In addition, bisulfite sequencing identified nine methylated CpG sites in the FUT3 core promoter (-500 similar to -206) region. Therein, the methylation level at the mC-9 site located in FUT3 showed a significantly negative association with mRNA expression (P < 0.05). A further dual-luciferase assay demonstrated that methylation at the mC-9 site of the FUT3 promoter inhibited its transcriptional activity. Then, we confirmed the binding of Spl to the FUT3 promoter using RNA knockdown and a ChIP-qPCR assay. Our findings indicate that DNA methylation at the mC-9 site may inhibit the binding of the transcription factor Spl, thus regulating the developmental expression of the FUT3 gene in the duodenum, providing some theoretical basis for the FUT3 gene as an important candidate marker of disease resistance in Meishan pigs.

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