您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

A new, simple and rapid HPLC method for determination of chlortetracycline in pig solid manure

文献类型： 外文期刊

作者： Wang, Ran 1 ; Wei, Ruicheng 1 ; Chen, Ming 1 ; Wang, Tian 2 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Food Safety, Jiangsu Key Lab Anim Derived Food Safety, Nanjing 210014, Jiangsu Prov, Peoples R China

2.Nanjing Agr Univ, Coll Anim Sci, Nanjing, Peoples R China

关键词： chlortetracycline: 57-62-5;pollutant;water pollutant solid manure;detection capacity;procedural specificity

期刊名称：ITALIAN JOURNAL OF ANIMAL SCIENCE （ 影响因子：2.217； 五年影响因子：2.376 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Chlortetracycline (CTC) is the most frequently used antibacterial in today's swine production for disease treatment and growth promotion. It is not absorbed completely and a considerable amount is excreted into manure when CTC is administrated to animal. For this reason there is high concern on potential risks that manure-derived CTC enters into the surface water and selects for resistant microorganism. In order to support the monitoring of CTC and study its fate in manure and environment, a simple and rapid analytical procedure was developed to determine chlortetracycline (CTC) in pig solid manure. The extraction solution, composed of acetone-4mol.L-1 hydrochloric acid-water (13: 1: 6, v/v/v), was mixed into the manure sample, and the solution was pH adjusted at 2.0-2.2 before extraction for 20 min. Chromatographic separation was carried out on a C-18 column at 375 nm with UV detector, using isocratic elution with oxalic acid-methanol-acetonitrile (72: 14: 14), v/v/v) as mobile phase at room temperature. Recoveries of CTC spiked samples at the levels of 1.0 mg.kg(-1), 2.0 mg.kg(-1) and 10.0 mg.kg(-1) were 92.2%-100.73% with relative standard deviation (RSD) of 2.81-7.11% intra-day and 1.343.25% inter-day, respectively. The limit of detection (LOD) and the limit of quantitation (LOQ) were 0.2 mg.kg(-1) and 0.8 mg.kg(-1), respectively. The procedure developed in this study was tested to be simple, sensitive and suitable for the determination and screening of CTC in pig solid manure samples.

  • 相关文献

[1]Removal of nutrients and veterinary antibiotics from swine wastewater by a constructed macrophyte floating bed system. Xian, Qiming,Chang, Zhizhou,Xian, Qiming,Hu, Lixia,Chen, Hancheng,Zou, Huixian.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台