文献类型： 外文期刊

作者： Jiang, LB ¹ ; Li, QL ² ; Li, MM ² ; Zhou, Z ² ; Wu, LG ² ; Fan, JH ³ ; Zhang, QQ; Zhu, HH; Xu, ZK;

作者机构： 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China

2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China; Shanghai Univ, Sch Life Sci, Shanghai 200436, Peoples R China; Shanghai Acad Agr Sci, Vet Sci Inst, Shanghai, Peoples R China

3.C

期刊名称：VACCINE （ 影响因子：3.641； 五年影响因子：3.816 ）

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收录情况： SCI

摘要： Based upon a mutant isolated from tobacco infected with a recombinant tobacco mosaic virus (TMV), a new TMV-based vector was developed in which four to six C-terminal amino acid residues were deleted from the viral coat protein (CP) subunit. The new vector was quite similar to the original TMV-based vector, which all expressed a well characterized epitope peptide F11 (P(142)-A(152)) of VP1 from foot-and-mouth disease virus (FMDV) serotype O in tobacco, in the infectivity, yield of the virus particles and more importantly protective activity of F11 in guinea pigs and swine against the FMDV. Furthermore, the capacity of the length of foreign peptide encoded by this new vector was much improved to successfully express a peptide F25 containing two fused epitopes F14 (R(200)-L(213)) and F11 of FMDV VP1, which was failed using the original vector in tobacco. Although animal assays indicated that such expressed F25 was not as efficient as F11 in the immunity, possibly due to lack of a spacer arm between the two fused epitopes, the new TMV-based vector may meet the requirement of expressing longer foreign peptides for different vaccines and other medicines.