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Oral immunization of mice with plant-derived fimbrial adhesin FaeG induces systemic and mucosal K88ad enterotoxigenic Escherichia coli-specific immune responses

文献类型： 外文期刊

作者： Liang, WQ 1 ; Huang, YH 2 ; Yang, XH 2 ; Zhou, Z 3 ; Pan, AH; Qian, BJ; Huang, C; Chen, JX; Zhang, DB;

作者机构： 1.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai 200240, Peoples R China

2.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai 200240, Peoples R China; Shanghai Acad Agr Sci, Agribiotech Res Ctr, Shanghai, Peoples R China; Nanjing Univ, Sch Life Sci, Nanjing 210008, Jiangsu, Peoples R China; Montana State Univ, Bozeman, MT 59717 USA; Shanghai Acad Agr Sci, Inst Anim & Vet Sci, Shanghai, Peoples R China

3.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai 200240, Peoples R China; Shanghai Acad Agr Sci, Agribiotech Res Ctr, Shanghai, Peoples R China; Nanjing Univ, Sch Life Sci, Nanjing 210008, Jiangsu, Peoples R China; Montana State Univ, Bozeman,

关键词： enterotoxigenic Escherichia coli;proteinaceous fimbriae;recombinant FaeG;transgenic tobacco

期刊名称：FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY （ 影响因子：3.078； 五年影响因子：2.747 ）

ISSN： 0928-8244

年卷期： 2006 年 46 卷 3 期

页码：

收录情况： SCI

摘要： The importance of adhesins in pathogenicity has resulted in them being useful targets in the defense against bacterial infections. To produce edible vaccines against piglet diarrhea caused by enterotoxigenic Escherichia coli (ETEC), plants were genetically engineered to produce recombinant fimbrial adhesin FaeG. To evaluate the efficacy of the edible vaccine FaeG in mice, the soluble protein extracts were examined by about 15 mu g recombinant FaeG for each oral immunization dose per mouse. After four doses of vaccination, both IgG and IgA antibodies specific to K88ad fimbriae were elicited in serum, and specific IgA antibodies were also evoked in feces of the immunized mice. Moreover, visible K88ad ETEC agglutination by the specific serum from the immunized mice was observed, implying the antibody was highly specific and effective. Results from an in vitro villous-adhesion assay further confirmed that serum antibodies of the immunized mice could inhibit K88ad ETEC from adhering to pig intestinal receptors, further demonstrating the oral immune efficacy of the plant-derived FaeG. This study provides a promising, noninvasive method for vaccinating swine by feeding supplements of transgenic plant. Moreover, the low cost and ease of delivery of this edible ETEC vaccine will facilitate its application in economically disadvantaged regions.

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