Duplication and expression analysis of multicopy miRNA gene family members in Arabidopsis and rice
文献类型： 外文期刊
作者： Jiang, DH 1 ; Yin, CS 2 ; Yu, AP 2 ; Zhou, XF 3 ; Liang, WQ; Yuan, Z; Xu, Y; Yu, QB; Wen, TQ; Zhang, DB;
作者机构： 1.Shanghai Jiao Tong Univ, SJTU SIBS PSU Joint Ctr Life Sci, Sch Life Sci & Biotechnol, Shanghai 200240, Peoples R China
2.Shanghai Jiao Tong Univ, SJTU SIBS PSU Joint Ctr Life Sci, Sch Life Sci & Biotechnol, Shanghai 200240, Peoples R China; Shanghai Univ, Sch Life Sci, Shanghai 200444, Peoples R China; Shanghai Normal Univ, Coll Life & Environm Sci, Shanghai 200234, Peoples R China; Shanghai Acad Agr Sci, Agro Biotech Res Ctr, Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China
3.Shanghai Jiao Tong Univ, SJTU SIBS PSU Joint Ctr Life Sci, Sch Life Sci & Biotechnol, Shanghai 200240, Peoples R China; Shanghai Univ, Sch Life Sci, Shanghai 200444
关键词： gene duplication;microRNA;multicopy
期刊名称：CELL RESEARCH （ 影响因子：25.617； 五年影响因子：25.924 ）
ISSN： 1001-0602
年卷期： 2006 年 16 卷 5 期
页码：
收录情况： SCI
摘要： To understand the expansion of multicopy microRNA (miRNA) families in plants, we localized the reported miRNA genes from Arabidopsis and rice to their chromosomes, respectively, and observed that 37% of 117 miRNA genes from Arabidopsis and 35% of 173 miRNA genes from rice were segmental duplications in the genome. In order to characterize whether the expression diversification has occurred among plant multicopy miRNA family members, we designed PCR primers targeting 48 predicted miRNA precursors from 10 families in Arabidopsis and rice. Results from RT-PCR data suggest that the transcribed precursors of members within the same miRNA family were present at different expression levels. In addition, although miR160 and miR162 sequences were conserved in Arabidopsis and rice, we found that the expression patterns of these genes differed between the two species. These data suggested that expression diversification has occurred in multicopy miRNA families, increasing our understanding of the expression regulation of miRNAs in plants.
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