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STM1863, a Member of the DUFs Protein Family, Is Involved in Environmental Adaptation, Biofilm Formation, and Virulence in Salmonella Typhimurium

文献类型： 外文期刊

作者： Ma, Zhongmei 1 ; Sun, Yaoqiang 1 ; Liu, Yuchen 2 ; Jiao, Jian 1 ; Li, Nengxiu 1 ; Zuo, Yufei 1 ; Li, Zhiyuan 1 ; Li, Yaling 1 ; Cai, Xuepeng 3 ; Meng, Qingling 1 ; Qiao, Jun 1 ;

作者机构： 1.Shihezi Univ, Coll Anim Sci & Technol, Shihezi, Xinjiang, Peoples R China

2.Xinjiang Acad Agr & Reclamat Sci, State Key Lab Sheep Genet Improvement & Hlth Prod, Shihezi, Xinjiang, Peoples R China

3.Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Lanzhou, Gansu, Peoples R China

4.Shihezi Univ, Coll Anim Sci & Technol, North St 4, Shihezi 832003, Xinjiang, Peoples R China

关键词： Salmonella Typhimurium; DUFs protein family; STM1863 gene; environmental stress; virulence

期刊名称：FOODBORNE PATHOGENS AND DISEASE （ 影响因子：2.8； 五年影响因子：3.2 ）

ISSN： 1535-3141

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Salmonella Typhimurium (STM) is an important zoonotic Gram-negative pathogen that can cause infection in a variety of livestock and poultry. Meanwhile, as an important foodborne pathogen, the bacterium can survive in various stressful environments and transmits through the fecal-oral route, posing a serious threat to global food safety. To investigate the roles of STM1863, a member of the DUFs protein family, involved in STM environmental adaptation, biofilm formation, and virulence. We analyzed the molecular characteristics of the protein encoded by STM1863 gene and examined intra- and extracellular expression levels of STM1863 gene in mouse macrophages. Furthermore, we constructed STM1863 gene deletion and complementation strains and determined its environmental adaptation under stressful conditions such as acid, alkali, high salt, bile salt, and oxidation. And the capacity of biofilm formation and pathogenicity of those strains were analyzed and compared. In addition, the interaction between the promoter of STM1863 gene and RcsB protein was analyzed using DNA gel electrophoresis migration assay (electrophoretic mobility shift assay [EMSA]). The experiments revealed that acid adaptability and biofilm formation ability of STM1863 gene deletion strain were significantly weakened compared with the parental and complementary strains. Moreover, the adhesion and invasion ability of STM1863 deletion strain to mouse macrophages was significantly decreased, while the median lethal dose (LD50) increased by 2.148-fold compared with the parental strain. In addition, EMSA confirmed that RcsB protein could bind to the promoter sequence of STM1863 gene, suggesting that the expression of STM1863 gene might be modulated by RcsB. The present study demonstrated for the first time that STM1863, a member of the DUFs protein family, is involved in the modulation of environmental adaptation, biofilm formation, and virulence.

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