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Expression profiles and characterization of microRNAs responding to chitin in Arthrobotrys oligospora

文献类型： 外文期刊

作者： Li, Ningxing 1 ; Sun, Yansen 1 ; Liu, Yucheng 2 ; Wei, Lixiang 1 ; Zhang, Jiahua 1 ; Li, Nengxiu 1 ; Sun, Dianming 1 ; Jiao, Jian 1 ; Zuo, Yufei 1 ; Li, Ruobing 1 ; Cai, Xuepeng 3 ; Qiao, Jun 1 ; Meng, Qingling 1 ;

作者机构： 1.Shihezi Univ, Coll Anim Sci & Technol, North St 4, Shihezi 832003, Xinjiang, Peoples R China

2.Xinjiang Acad Agr & Reclamat Sci, Inst Anim Husb & Vet Sci, State Key Lab Sheep Genet Improvement & Hlth Breed, Shihezi 832000, Xinjiang, Peoples R China

3.Chinese Acad Agr Sci, State Key Lab Vet Etiol Biol, Lanzhou Vet Res Inst, Lanzhou 730046, Gansu, Peoples R China

关键词： Arthrobotrys oligospora; Chitin-responsive miRNAs; Regulation of gene expression; Chitinase

期刊名称：ARCHIVES OF MICROBIOLOGY （ 影响因子：2.8； 五年影响因子：2.8 ）

ISSN： 0302-8933

年卷期： 2024 年 206 卷 5 期

页码：

收录情况： SCI

摘要： Extracellular proteases, such as chitinases secreted by Arthrobotrys oligospora (A. oligospora), play a crucial role in the process of nematode infection. However, post-transcriptional regulation of gene expression involving microRNAs (miRNAs) in A. oligospora remains scarcely described. Hereto, transcriptome sequencing was carried out to analyze the expression profiles of chitin-responsive miRNAs in A. oligospora. Based on the RNA-seq data, the differential expression of miRNAs (DEmiRNAs) in response to chitin was screened, identified and characterized in A. oligospora. Meanwhile, the potential target genes were predicted by the online tools miRanda and Targetscan, respectively. Furthermore, the interaction of DEmiRNA with it's target gene was validated by a dual-luciferase reporter assay system. Among 85 novel miRNAs identified, 25 miRNAs displayed significant differences in expression in A. oligospora in response to chitin. Gene Ontology (GO) analysis showed that the potential genes targeted by DEmiRNAs were enriched in the biological processes such as bio-degradation, extracellular components and cell cycle. KEGG analysis revealed that the target genes were mainly involved in Hippo, carbon and riboflavin metabolic pathway. Outstandingly, chitinase AOL_s00004g379, which is involved in the hydrolysis metabolic pathway of chitin, was confirmed to be a target gene of differential miR_70. These findings suggest that chitin-responsive miRNAs are involved in the regulation of cell proliferation, predator hyphae growth and chitinase expression through the mechanisms of post-transcriptional regulation, which provides a new perspective to the molecular mechanisms underlying miRNAs-mediated control of gene expression in A. oligospora.

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