文献类型： 外文期刊

作者： Hai, Guiping ¹ ; Bai, Jiachen ² ; Liu, Yucheng ² ; Li, Jun ³ ; Liu, Aiju ⁴ ; Wang, Jingjing ² ; Liu, Qian ⁴ ; Liu, Weijun ¹ ; Wan, Pengcheng ² ; Fu, Xiangwei ⁴ ;

作者机构： 1.Xinjiang Agr Univ, Coll Anim Sci, Urumqi, Peoples R China

2.Xinjiang Acad Agr & Reclamat Sci, Inst Anim Husb & Vet Sci, State Key Lab Sheep Genet Improvement & Hlth Breed, Shihezi, Peoples R China

3.Hebei Med Univ, Reprod Med Ctr, Dept Reprod Med, Hosp 1, Shijiazhuang 050031, Peoples R China

4.China Agr Univ, Coll Anim Sci & Technol, Natl Engn Lab Anim Breeding, Key Lab Anim Genet Breeding & Reprod MARA,Beijing, Beijing, Peoples R China

关键词： nanoparticles; PLGA-RES; oocytes; endocytosis; vitrification

期刊名称：FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY （ 影响因子：5.7； 五年影响因子：6.2 ）

ISSN： 2296-4185

年卷期： 2024 年 12 卷

页码：

收录情况： SCI

摘要： Irreversible cryogenic damage caused by oocyte vitrification limits its widespread use in female fertility preservation. In recent years, nanoparticles (NPs) have gained great attention as potential alternatives in protecting oocytes against cryoinjuries. In this paper, a novel composite nanoparticle, poly (lactic-co-glycolic acid)-resveratrol (PLGA-RES) was designed to improve the biocompatibility and sustained release properties by encapsulating natural antioxidant RES into PLGA NPs. Firstly, biotoxicity and oxidation resistance of PLGA-RES were determined, and the results showed that PLGA-RES had nontoxic effect on oocyte survival during in vitro maturation (IVM) (97.08% +/- 0.24% vs. 98.89% +/- 1.11%, p > 0.05). Notably, PLGA-RES even increased maturation (65.10% +/- 4.11% vs. 52.85% +/- 2.87%, p < 0.05) and blastocyst rate (56.13% +/- 1.36% vs. 40.91% +/- 5.85%, p < 0.05). Moreover, the reduced reactive oxygen species (ROS) level (13.49 +/- 2.30 vs. 34.07 +/- 3.30, p < 0.01), increased glutathione (GSH) (44.13 +/- 1.57 vs. 37.62 +/- 1.79, p < 0.01) and elevated mitochondrial membrane potential (MMP) levels (43.10 +/- 1.81 vs. 28.52 +/- 1.25, p < 0.01) were observed in oocytes treated with PLGA-RES when compared with that of the control group. Subsequently, the role of PLGA-RES played in oocytes during vitrification was systematically evaluated. The results showed that the addition of PLGA-RES during vitrification and thawing significantly improved the survival rate (80.42% +/- 1.97% vs. 75.37% +/- 1.3%, p < 0.05). Meanwhile, increased GSH (15.09 +/- 0.86 vs. 14.51 +/- 0.78, p < 0.01) and mitochondrial membrane potential (22.56 +/- 3.15 vs. 6.79 +/- 0.60, p < 0.01), decreased reactive oxygen species levels (52.11 +/- 2.95 vs. 75.41 +/- 7.23, p < 0.05) and reduced mitochondrial abnormality distribution rate (25.00% +/- 0.29% vs. 33.33% +/- 1.15%, p < 0.01) were assessed in vitrified MII oocytes treated with PLGA-RES. Furthermore, transcriptomic analyses demonstrated that PLGA-RES participated in endocytosis and PI3K/AKT/mTOR pathway regulation, which was verified by the rescued expression of ARRB2 and ULK3 protein after PLGA-RES treatment. In conclusion, PLGA-RES exhibited potent antioxidant activity, and could be used as an efficacious strategy to improve the quality of vitrified oocytes.