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Metformin alleviates cryoinjuries in porcine oocytes by reducing membrane fluidity through the suppression of mitochondrial activity

文献类型： 外文期刊

作者： Zhou, Dan 1 ; Liu, Hongyu 1 ; Zheng, Lv 1 ; Liu, Aiju 1 ; Zhuan, Qingrui 1 ; Luo, Yuwen 1 ; Zhou, Guizhen 1 ; Meng, Lin 2 ; Hou, Yunpeng 2 ; Wu, Guoquan 3 ; Li, Jun 4 ; Fu, Xiangwei 1 ;

作者机构： 1.China Agr Univ, Coll Anim Sci & Technol, Natl Engn Lab Anim Breeding,State Key Lab Anim Bio, Key Lab Anim Genet Breeding & Reprod MARA,Beijing, Beijing, Peoples R China

2.China Agr Univ, Coll Biol Sci, State Key Lab Anim Biotech Breeding, Beijing, Peoples R China

3.Yunnan Anim Sci & Vet Inst, Yunnan Prov Engn Lab Anim Genet Resource Conservat, Kunming, Peoples R China

4.Hebei Med Univ, Hosp 1, Reprod Med Ctr, Dept Reprod Med, Shijiazhuang, Peoples R China

5.Xinjiang Acad Agr & Reclamat Sci, State Key Lab Sheep Genet Improvement & Hlth Breed, Shihezi, Peoples R China

期刊名称：COMMUNICATIONS BIOLOGY （ 影响因子：5.2； 五年影响因子：5.6 ）

ISSN：

年卷期： 2024 年 7 卷 1 期

页码：

收录情况： SCI

摘要： Plasma membrane damage in vitrified oocytes is closely linked to mitochondrial dysfunction. However, the mechanism underlying mitochondria-regulated membrane stability is not elucidated. A growing body of evidence indicates that mitochondrial activity plays a pivotal role in cell adaptation. Since mitochondria work at a higher temperature than the constant external temperature of the cell, we hypothesize that suppressing mitochondrial activity would protect oocytes from extreme stimuli during vitrification. Here we show that metformin suppresses mitochondrial activity by reducing mitochondrial temperature. In addition, metformin affects the developmental potential of oocytes and improves the survival rate after vitrification. Transmission electron microscopy results show that mitochondrial abnormalities are markedly reduced in vitrified oocytes pretreated with metformin. Moreover, we find that metformin transiently inhibits mitochondrial activity. Interestingly, metformin pretreatment decreases cell membrane fluidity after vitrification. Furthermore, transcriptome results demonstrate that metformin pretreatment modulates the expression levels of genes involved in fatty acid elongation process, which is further verified by the increased long-chain saturated fatty acid contents in metformin-pretreated vitrified oocytes by lipidomic profile analysis. In summary, our study indicates that metformin alleviates cryoinjuries by reducing membrane fluidity via mitochondrial activity regulation. In this study, the authors show that metformin, a drug primarily used for the treatment of diabetes, provides protection against cryoinjuries in porcine oocytes by reducing membrane fluidity and mitochondrial activity.

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