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Development of an immunochromatographic assay for the rapid detection of chlorpyrifos-methyl in water samples

文献类型： 外文期刊

作者： Hua, Xiude 1 ; Qian, Guoliang 1 ; Yang, Jifei; Hu, Baishi 1 ; Fan, Jiaqin 1 ; Qin, Na 1 ; Li, Gang 1 ; Wang, Yuyan 1 ; Li 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Chinese Minist Agr, Key Lab Monitoring & Management Plant Dis & Insec, Nanjing 210095, Peoples R China

2.Nanjing Agr Univ, Coll Plant P

关键词： Chlorpyrifos-methyl;Colloidal gold;Gold immunochromatographic assay;One-step strip test;Pesticide residue

期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：10.618； 五年影响因子：9.323 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A rapid (less than 10min), qualitative and semi-quantitative immunochromatography using colloidal gold-antibody probe was successfully developed and applied in determination of chlorpyrifos-methyl (a wide-spectrum organophosphorus pesticide) in water samples. The qualitative detection limit of chlorpyrifos-methyl was determined as 0.6μgml~(-1) by using immunochromatography. In the semi-quantitative experiment, the detection results of chlorpyrifos-methyl were scanned by a membrane strip reader, and a detection curve representing the scanned data average was obtained. After conversion, it was observed that in the range of 50-12,150ngml~(-1), the graph between logit(B/B_0) and logarithm of concentration of chlorpyrifos-methyl was linear, from which, the regression equation (y=-2.5229x+7.5951, R~2=0.9889) and IC_(50) value (1024.39ngml~(-1)) was obtained, respectively. Meanwhile, the detection limit was calculated as 132.91ngml~(-1) by the extrapolation of B_0-2SD. In addition, the cross-reactivities were less than 1% with tested analog compounds and regarded as negligible. The recoveries obtained by standard chlorpyrifos-methyl addition to water samples were 102.5-107.6%. Overall, to our knowledge, this is the first report of qualitative and semi-quantitative detection of chlorpyrifos-methyl by immunochromatography.

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