您好，欢迎访问河南省农业科学院 机构知识库！
河南省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Development of an immunochromatographic assay for the rapid detection of chlorpyrifos-methyl in water samples

文献类型： 外文期刊

作者： Hua, Xiude 1 ; Qian, Guoliang 1 ; Yang, Jifei; Hu, Baishi 1 ; Fan, Jiaqin 1 ; Qin, Na 1 ; Li, Gang 1 ; Wang, Yuyan 1 ; Li 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Chinese Minist Agr, Key Lab Monitoring & Management Plant Dis & Insec, Nanjing 210095, Peoples R China

2.Nanjing Agr Univ, Coll Plant P

关键词： Chlorpyrifos-methyl;Colloidal gold;Gold immunochromatographic assay;One-step strip test;Pesticide residue

期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：10.618； 五年影响因子：9.323 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A rapid (less than 10min), qualitative and semi-quantitative immunochromatography using colloidal gold-antibody probe was successfully developed and applied in determination of chlorpyrifos-methyl (a wide-spectrum organophosphorus pesticide) in water samples. The qualitative detection limit of chlorpyrifos-methyl was determined as 0.6μgml~(-1) by using immunochromatography. In the semi-quantitative experiment, the detection results of chlorpyrifos-methyl were scanned by a membrane strip reader, and a detection curve representing the scanned data average was obtained. After conversion, it was observed that in the range of 50-12,150ngml~(-1), the graph between logit(B/B_0) and logarithm of concentration of chlorpyrifos-methyl was linear, from which, the regression equation (y=-2.5229x+7.5951, R~2=0.9889) and IC_(50) value (1024.39ngml~(-1)) was obtained, respectively. Meanwhile, the detection limit was calculated as 132.91ngml~(-1) by the extrapolation of B_0-2SD. In addition, the cross-reactivities were less than 1% with tested analog compounds and regarded as negligible. The recoveries obtained by standard chlorpyrifos-methyl addition to water samples were 102.5-107.6%. Overall, to our knowledge, this is the first report of qualitative and semi-quantitative detection of chlorpyrifos-methyl by immunochromatography.

  • 相关文献

[1]Development of an immunochromatographic test strip for simultaneous qualitative and quantitative detection of ochratoxin A and zearalenone in cereal. Sun, Yaning,Xing, Guangxu,Yang, Jifei,Wang, Fangyu,Deng, Ruiguang,Zhang, Gaiping,Hu, Xiaofei,Sun, Yaning,Zhang, Yong,Zhang, Gaiping.

[2]Rapid and Sensitive Detection of the Food Allergen Glycinin in Powdered Milk Using a Lateral Flow Colloidal Gold Immunoassay Strip Test. Wang, Yao,Li, Zhixi,Deng, Ruiguang,Zhang, Gaiping,Li, Qingmei,Yang, Jifei,Sun, Yaning,Hu, Xiaofei,Zhang, Gaiping.

[3]Development of a Lateral Flow Colloidal Gold Immunoassay Strip for the Rapid Detection of Olaquindox Residues. Song, Chunmei,Liu, Qingtang,Zhi, Aimin,Yang, Jifei,Zhi, Yubao,Li, Qingmei,Hu, Xiaofei,Deng, Ruiguang,Zhang, Gaiping,Song, Chunmei,Casas, Justin,Tang, Liang.

[4]Establishment of a Lateral Flow Colloidal Gold Immunoassay Strip for the Rapid Detection of Soybean Allergen beta-Conglycinin. Wang, Yao,Li, Zhaozhou,Pei, Yafeng,Hou, Yuze,Wang, Yao,Li, Qingmei,Sun, Yaning,Yang, Jifei,Yang, Yanyan,Zhi, Yubao,Deng, Ruiguang,Hu, Xiaofei.

[5]Establishment of a lateral flow colloidal gold immunoassay strip for the rapid detection of estradiol in milk samples. Yang, Xing-dong,Wang, Fang-yu,Zhang, Gai-ping,Yang, Xing-dong,Zeng, Xian-yin,Song, Chun-mei,Wu, Shu-yan,Zhang, Gai-ping.

[6]Colloidal gold-McAb probe-based rapid immunoassay strip for simultaneous detection of fumonisins in maize. Yao, Jingjing,Sun, Yaning,Li, Qingmei,Wang, Fangyu,Teng, Man,Yang, Yanyan,Deng, Ruiguang,Hu, Xiaofei.

[7]Development of a colloidal gold-based strip test for the detection of chlorothalonil residues in cucumber. Yang, Xingdong,Zeng, Xianyin,Yang, Xingdong,Zhang, Gaiping,Wang, Fangyu,Wang, Yinbiao,Hu, Xiaofei,Li, Qingmei,Jia, Guochao,Liu, Zhen,Wang, Yao,Deng, Ruiguang,Zhang, Gaiping.

[8]Development of a Lateral Flow Colloidal Gold Immunoassay Strip for the Rapid Detection of Enrofloxacin Residues. Zhao, Yinli,Zhang, Gaiping,Liu, Qingtang,Teng, Man,Yang, Jifei,Zhao, Yinli,Wang, Jianhua.

[9]Development of an Immunochromatographic Strip Test for the Rapid Detection of Zearalenone in Corn. Sun, Yaning,Hu, Xiaofei,Yang, Jifei,Wang, Fangyu,Wang, Yao,Deng, Ruiguang,Zhang, Gaiping,Sun, Yaning,Zhang, Yong,Zhang, Gaiping.

[10]Development of an immunochromatographic lateral flow test strip for detection of beta-adrenergic agonist Clenbuterol residues. Zhang, GP,Wang, XN,Yang, JF,Yang, YY,Xing, GX,Li, QM,Zhao, D,Chai, SJ,Guo, JQ. 2006

[11]Development of a lateral-flow immunochromatographic test device for the rapid detection of difloxacin residues. Zhi, Ai-min,Li, Bin-bin,Liu, Qing-tang,Hu, Xiao-fei,Zhao, Dong,Hou, Yu-ze,Deng, Rui-guang,Zhang, Gai-ping,Li, Bin-bin,Chai, Shu-Jun. 2010

[12]Development of a lateral flow immunoassay strip for screening of sulfamonomethoxine residues. Zhang, G.,Wang, X.,Bao, Y.,Wang, Z.,Zhang, G.,Wang, X.,Yang, Y.,Luo, J.,Li, Q.,Guo, J.,Yang, J.,Xing, G.,Chai, S.,Shi, T.,Liu, Q.,Zhi, A.,Qu, M.. 2008

[13]Development of a one-step strip test for the diagnosis of chicken infectious bursal disease. Zhang, GP,Li, QM,Yan, YY,Guo, JQ,Li, XW,Deng, RG,Xiao, ZJ,Xing, GX,Yang, JF,Zhao, D,Cai, SJ,Zang, WM. 2005

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：河南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台