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An immunochromatographic strip sensor for marbofloxacin residues

文献类型： 外文期刊

作者： Yang, Xingdong 1 ; Li, Qingmei 2 ; Kwee, Sharon 3 ; Yang, Jifei 2 ; Zhang, Qianqian 1 ; Hu, Xiaofei 2 ;

作者机构： 1.Zhoukou Normal Univ, Inst Food & Drug Inspect, Zhoukou, Peoples R China

2.Henan Acad Agr Sci, Key Lab Anim Immunol, Henan Prov Key Lab Anim Immunol, Minist Agr, Zhengzhou, Peoples R China

3.Univ Texas San Antonio, Dept Biomed Engn, San Antonio, TX USA

期刊名称：PLOS ONE （ 影响因子：3.7； 五年影响因子：3.8 ）

ISSN： 1932-6203

年卷期： 2024 年 19 卷 3 期

页码：

收录情况： SCI

摘要： Marbofloxacin (MBF) was once widely used as a veterinary drug to control diseases in animals. MBF residues in animal food endanger human health. In the present study, an immunochromatographic strip assay (ICSA) utilizing a competitive principle was developed to rapidly detect MBF in beef samples. The 50% inhibitory concentration (IC50) and the limit of detection (LOD) of the ICSAs were 2.5 ng/mL and 0.5 ng/mL, respectively. The cross-reactivity (CR) of the MBF ICSAs to Ofloxacin (OFL), enrofloxacin (ENR), norfloxacin (NOR), and Ciprofloxacin (CIP) were 60.98%, 32.05%, 22.94%, and 23.58%, respectively. The CR for difloxacin (DIF) and sarafloxacin (SAR) was less than 0.1%. The recovery rates of MBF in spiked beef samples ranged from 82.0% to 90.4%. The intra-assay and interassay coefficients of variation (CVs) were below 10%. In addition, when the same authentic beef samples were detected in a side-by-side comparison between the ICSAs and HPLC-MS, no statistically significant difference was observed. Therefore, the proposed ICSAs can be a useful tool for monitoring MBF residues in beef samples in a qualitative and quantitative manner.

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